Hordhac ku saabsan Maaddooyinka Yar ee Peptide-ka ee raadkooda
Qaybta 1aad Taariikhda Waxyaabaha Lagu Daro Macdanta Raadka
Waxaa loo qaybin karaa afar jiil iyadoo loo eegayo horumarinta waxyaabaha lagu daro macdanta raadadka ah:
Jiilkii ugu horreeyay: Cusbo aan dabiici ahayn oo macdanta raadadka ah, sida copper sulfate, ferrous sulfate, zinc oxide, iwm; Jiilkii labaad: Cusbo aashito dabiici ah oo macdanta raadadka ah, sida ferrous lactate, ferrous fumarate, copper citrate, iwm.; Jiilkii saddexaad: Amino acid chelate heerka quudinta ee macdanta raadadka ah, sida zinc methionine, iron glycine iyo zinc glycine; Jiilkii afraad: Cusbo borotiin iyo cusbo peptide chelating yar oo macdanta raadadka ah, sida borotiin copper, protein iron, protein zinc, protein manganese, peptide copper yar, peptide iron yar, peptide zinc yar, peptide manganese yar, iwm.
Jiilka koowaad waa macdanno aan dabiici ahayn, jiilka labaad ilaa afraadna waa macdanno dabiici ah.
Qaybta 2aad Maxaad u Dooranaysaa Chelates-ka Peptide-ka Yar
Chelates-ka peptide-ka yar wuxuu leeyahay waxtarka soo socda:
1. Marka peptide-yada yaryar ay la jaanqaadaan ion-yada birta ah, waxay qani ku yihiin qaabab badan waxayna adag tahay in la dherego;
2. Kuma tartanto kanaalada amino acid, waxay leedahay goobo nuugis badan iyo xawaare nuugis oo degdeg ah;
3. Isticmaalka tamarta oo yaraaday; 4. Dhigaal badan, heerka isticmaalka oo sarreeya iyo waxqabadka wax soo saarka xoolaha oo si weyn loo hagaajiyay;
5. Ka-hortagga bakteeriyada iyo antioxidant-ka;
6. Xakamaynta difaaca jirka.
Tiro badan oo daraasado ah ayaa muujiyay in sifooyinka kor ku xusan ama saamaynta chelates-ka yar ee peptide ay ka dhigayaan inay yeeshaan rajo ballaaran oo ku saabsan codsigooda iyo awooddooda horumarineed, sidaa darteed shirkaddayadu ugu dambeyntii waxay go'aansatay inay qaadato chelates-ka yar ee peptide iyadoo diiradda saareysa cilmi-baarista iyo horumarinta wax soo saarka macdanta raad-raaca dabiiciga ah ee shirkadda.
Qaybta 3aad Waxtarka chelates-ka yar yar ee peptide-ka
1. Xiriirka ka dhexeeya peptides, amino acids iyo borotiinada
Miisaanka molecular ee borotiinku waa in ka badan 10000;
Miisaanka molecular ee peptide waa 150 ~ 10000;
Peptides-ka yaryar, oo sidoo kale loo yaqaan peptides-ka yaryar ee molecular-ka, waxay ka kooban yihiin 2 ~ 4 amino acids;
Celceliska miisaanka molecular ee amino acids waa qiyaastii 150.
2. Isku-dubaridka kooxaha amino acids iyo peptides chelated oo leh biraha
(1) Kooxaha isku-dubaridka ee asiidhyada amino
Kooxaha isku-dubaridka ee amino acids-ka:
Kooxaha amino iyo carboxyl ee ku jira a-carbon;
Kooxaha silsiladaha dhinaca ee qaar ka mid ah asiidhyada a-amino, sida kooxda sulfhydryl ee cysteine, kooxda phenolic ee tyrosine iyo kooxda imidazole ee histidine.
(2) Kooxaha isku-dubaridka ee peptides-ka yaryar
Peptides-ka yaryar waxay leeyihiin kooxo isku-dubbaridan oo ka badan amino acids-ka. Marka ay chelate ku sameeyaan ion-yada birta ah, way fududahay in la chelate, waxayna samayn karaan chelation badan, taasoo ka dhigaysa chelate-ka mid deggan.
3. Waxtarka badeecada chelate-ka yar ee peptide
Saldhigga aragtida ee peptide yar oo kor u qaadaya nuugista macdanta raadadka
Astaamaha nuugista ee peptides-ka yaryar ayaa ah saldhigga aragtiyeed ee lagu dhiirrigelinayo nuugista walxaha raadadka. Sida laga soo xigtay aragtida dheef-shiid kiimikaadka borotiinka ee dhaqameed, waxa xayawaanku u baahan yihiin borotiinku waa waxa ay u baahan yihiin amino acids-ka kala duwan. Si kastaba ha ahaatee, sannadihii ugu dambeeyay, daraasaduhu waxay muujiyeen in saamiga isticmaalka ee amino acids-ka ee quudinta ilo kala duwan uu ka duwan yahay, marka xayawaanka lagu quudiyo cunto homozygous ah ama cunto dheellitiran oo amino acid ah oo borotiin hooseeya, waxqabadka wax soo saarka ugu fiican lama heli karo (Baker, 1977; Pinchasov et al., 1990) [2,3]. Sidaa darteed, aqoonyahanno qaar ayaa soo bandhigay aragtida ah in xayawaanku ay leeyihiin awood nuugis gaar ah oo loogu talagalay borotiinka buuxa laftiisa ama peptides-ka la xiriira. Agar(1953)[4] markii ugu horreysay waxay arkeen in marinnada xiidmaha ay si buuxda u nuugi karaan oo ay qaadi karaan diglycidyl. Tan iyo markaas, cilmi-baarayaashu waxay soo bandhigeen dood qancin leh oo ah in peptides-ka yaryar si buuxda loo nuugi karo, iyagoo xaqiijinaya in glycylglycine-ka buuxa la qaado oo la nuugo; Tiro badan oo peptides-ka yaryar ah ayaa si toos ah loogu nuugi karaa wareegga nidaamka qaab peptides ah. Hara et al. (1984)[5] sidoo kale waxay tilmaameen in wax soo saarka dhammaadka dheefshiidka borotiinku ee ku jira habka dheefshiidka ay yihiin peptides yaryar halkii ay ka ahaan lahaayeen amino acids xor ah (FAA). Peptides-ka yaryar waxay si buuxda uga gudbi karaan unugyada xuubka mindhicirka oo ay geli karaan wareegga nidaamka (Le Guowei, 1996)[6].
Horumarka Cilmi-baarista ee Peptide-ka Yar ee Horumarinta Nuugista Macdanta Dabiiciga ah, Qiao Wei, iwm.
Chelate-yada peptide-ka yar yar ayaa la qaadaa oo lagu nuugaa qaab peptides yaryar ah
Sida laga soo xigtay habka nuugista iyo gaadiidka iyo astaamaha peptides-ka yaryar, macdanta raadku waxay la jaanqaadaan peptides-ka yaryar maadaama ay yihiin ligands-ka ugu muhiimsan guud ahaan, taas oo aad ugu habboon horumarinta awoodda bayoolojiga ee macdanta raadku. (Qiao Wei, et al)
Waxtarka Chelates-ka Peptide-ka Yar
1. Marka peptide-yada yaryar ay la jaanqaadaan ion-yada birta ah, waxay qani ku yihiin qaabab badan waxayna adag tahay in la dherego;
2. Kuma tartanto kanaalada amino acid, waxay leedahay goobo nuugis badan iyo xawaare nuugis oo degdeg ah;
3. Isticmaalka tamarta oo yaraada;
4. Dhigaal badan, heerka isticmaalka oo sarreeya iyo waxqabadka wax soo saarka xoolaha oo si weyn loo hagaajiyay;
5. Ka-hortagga bakteeriyada iyo antioxidant-ka; 6. Nidaaminta difaaca jirka.
4. Faham dheeraad ah oo ku saabsan peptides-ka
Labada qof ee isticmaala peptide kee baa lacag badan ku bixiya?
- Peptide-ka isku xidhka
- Fosfopeptide
- Waxyaabaha la xiriira
- Peptide-ka lidka ku ah jeermiska
- Peptide-ka difaaca jirka
- Neuropeptide
- Peptide-ka hoormoonka
- Peptide-ka ka hortagga oksaydheynta
- Peptides-ka nafaqada leh
- Peptides-ka xawaashka leh
(1) Kala soocidda peptides-ka
(2) Saamaynta fiisiyoolajiyadeed ee peptides-ka
- 1. Hagaaji dheelitirka biyaha iyo elektrolytka ee jirka;
- 2. Samaynta unugyada difaaca jirka ee ka hortaga bakteeriyada iyo caabuqyada si habdhiska difaaca jirka uu u horumariyo shaqada difaaca jirka;
- 3. Kor u qaadida bogsashada nabarrada; Dayactir degdeg ah oo ku yimaada dhaawaca unugyada epithelial.
- 4. Samaynta enzymes-ka jirka waxay ka caawisaa in cuntada loo beddelo tamar;
- 5. Hagaaji unugyada, hagaaji dheef-shiid kiimikaadka unugyada, ka hortagga burburka unugyada, iyo door ka ciyaar ka hortagga kansarka;
- 6. Kor u qaadida isku-darka iyo nidaaminta borotiinka iyo ensaymada;
- 7. Farriin kiimiko oo muhiim ah oo gudbisa macluumaadka unugyada iyo xubnaha;
- 8. Ka hortagga cudurrada wadnaha iyo xididdada dhiigga;
- 9. Nidaami habdhiska endocrine iyo neerfayaasha.
- 10. Hagaajinta habdhiska dheefshiidka iyo daaweynta cudurrada caloosha iyo mindhicirka ee daba dheeraada;
- 11. Hagaajinta macaanka, laabjeexa, laabjeexa iyo cudurrada kale.
- 12. Caabuqa fayraska, ka hortagga gabowga, baabi'inta xagjirrada xorta ah ee xad-dhaafka ah ee jirka.
- 13. Kor u qaad shaqada dhiigga, daawee dhiig-yarida, ka hortagga isku-darka platelet-ka, kaas oo hagaajin kara awoodda qaadista oksijiinta ee unugyada dhiigga cas.
- 14. Si toos ah ula dagaallan fayraska DNA-da iyo bartilmaameedsiga bakteeriyada fayraska.
5. Shaqada nafaqada ee laba-jibbaaran ee chelates-ka yar yar
Chelate-ka yar ee peptide-ku wuxuu galaa unugga guud ahaan jidhka xayawaanka, iyoka dibna si toos ah ayuu u jebiyaa xiriirka chelation-kaUnugga dhexdiisa wuxuuna u kala baxaa peptide iyo biraha, kuwaas oo si kala duwan loo isticmaaloxayawaanku si uu u ciyaaro laba hawlood oo nafaqo ahgaar ahaan,doorka shaqada ee peptide.
Shaqada peptide yar
- 1. Kor u qaadida sameynta borotiinka ee unugyada murqaha xayawaanka, yaraynta apoptosis, iyo kor u qaadida koritaanka xayawaanka
- 2. Hagaajinta qaab-dhismeedka dhirta xiidmaha iyo kor u qaadida caafimaadka xiidmaha
- 3. Bixi qalfoof kaarboon ah oo kordhiya firfircoonida ensaymada dheefshiidka sida amylase-ka xiidmaha iyo protease
- 4. Waxay leedahay saameyn ka hortagta oksaydhka
- 5. Waxay leedahay sifooyin ka hortagga bararka
- 6……
6. Faa'iidooyinka chelates-ka yar ee peptide marka loo eego chelates-ka amino acid
| Macdanta raadadka ee amino acid chelated | Macdanta yar yar ee peptide chelated | |
| Qiimaha alaabta ceeriin ah | Walxaha ceeriin ee amino acid-ka keliya ayaa qaali ah | Qalabka ceeriin ee keratin ee Shiinaha waa mid aad u badan. Timaha, qoobabka iyo geesaha xoolaha iyo qashinka borotiinka iyo haraaga maqaarka ee warshadaha kiimikada waa walxo cayriin oo tayo sare leh oo raqiis ah. |
| Saamaynta nuugista | Kooxaha amino iyo carboxyl waxay isku mar ku lug leeyihiin isku-darka amino acids iyo curiyayaasha birta, iyagoo sameeya qaab-dhismeed endocannabinoid baaskiil-wadareed oo la mid ah kan dipeptides-ka, iyadoo aysan jirin kooxo carboxyl bilaash ah oo ku jira, kuwaas oo kaliya lagu nuugo karo nidaamka oligopeptide. (Su Chunyang et al., 2002) | Marka peptide-yada yaryar ay ka qayb qaataan chelation-ka, qaab-dhismeedka hal giraan oo chelation ah ayaa guud ahaan sameeya kooxda amino-ka ee terminal-ka iyo oksijiinta isku xidhka peptide ee ku xigta, chelate-kuna wuxuu hayaa koox carboxyl bilaash ah, taas oo lagu nuugi karo nidaamka dipeptide-ka, iyadoo leh xoog nuugis oo aad uga sarreeya nidaamka oligopeptide. |
| Xasilloonida | Aayoon bir ah oo leh hal ama in ka badan oo giraangiraha shan-xubnood leh ama lix-xubnood leh oo ka kooban kooxaha amino, kooxaha carboxyl, kooxaha imidazole, kooxaha phenol, iyo kooxaha sulfhydryl. | Marka laga soo tago shan kooxood oo isku-dubarid ah oo amino acids ah, kooxaha carbonyl iyo imino ee ku jira peptides-ka yaryar ayaa sidoo kale ku lug yeelan kara isku-dubaridka, taasoo ka dhigaysa peptide-yada yaryar inay ka xasilloon yihiin chelates-ka amino acid. (Yang Pin et al., 2002) |
7. Faa'iidooyinka peptide-ka yar marka loo eego glycolic acid iyo methionine chelates
| Macdanta raadadka Glycine chelated | Macdanta raadadka methionine chelated | Macdanta yar yar ee peptide chelated | |
| Foomka isku-dubaridka | Kooxaha carboxyl iyo amino ee glycine waxaa loo habeyn karaa ayoonada birta. | Kooxaha carboxyl iyo amino ee methionine waxaa loo habeyn karaa ayoonada birta ah. | Marka lagu daro ayoonnada birta ah, waxay qani ku tahay qaababka isku-dubbaridka mana aha mid si fudud loo buuxin karo. |
| Shaqada nafaqada | Noocyada iyo shaqooyinka amino acids-ka waa hal. | Noocyada iyo shaqooyinka amino acids-ka waa hal. | Thenoocyo kala duwan oo qani ahamino acids-ka waxay bixiyaan nafaqo dhammaystiran, halka peptide-yada yaryar ay si habboon u shaqayn karaan. |
| Saamaynta nuugista | Glycine chelates waxay leeyihiin walxonoKooxaha carboxyl ee bilaashka ah ayaa jira waxayna leeyihiin saameyn nuugis gaabis ah. | Methionine chelates waxay leeyihiinnoKooxaha carboxyl ee bilaashka ah ayaa jira waxayna leeyihiin saameyn nuugis gaabis ah. | Chelate-yada yar yar ee peptide-ka ayaa sameysmayku jirajoogitaanka kooxaha carboxyl ee bilaashka ah waxayna leeyihiin saameyn nuugis oo degdeg ah. |
Qaybta 4 Magaca Ganacsiga "Chelates-ka Peptide-ka yar"
Chelates-ka Peptide-macdanta yar, sida magacu tilmaamayo, way fududahay in la kala saaro.
Waxay tilmaamaysaa ligands yar yar oo peptide ah, kuwaas oo aan si fudud u buuxsamin sababtoo ah tirada badan ee kooxaha isku-dubaridka, Way fududahay in la sameeyo chelate badan oo leh walxo bir ah, oo leh xasillooni wanaagsan.
Qaybta 5aad Hordhac ku saabsan Badeecadaha Taxanaha Peptide-macdanta Yar
1. Naxaasta chelated-ka ah ee macdanta yar ee peptide (magaca ganacsiga: Copper Amino Acid Chelate Feed Grade)
2. Birta chelated-ka ah ee macdanta yar ee peptide raad yar (magaca ganacsiga: Ferrous Amino Acid Chelate Feed Grade)
3. Zinc chelated mineral trace peptide yar (magaca ganacsiga: Zinc Amino Acid Chelate Feed Grade)
4. Manganese chelated oo ah macdan yar oo peptide ah (magaca ganacsiga: Manganese Amino Acid Chelate Feed Grade)
Fasalka Quudinta Chelate ee Copper Amino Acid
Darajada Quudinta Chelate ee Ferrous Amino Acid
Darajada Quudinta Chelate ee Zinc Amino Acid
Darajada Quudinta Chelate ee Manganese
1. Darajada Quudinta Chelate ee Copper Amino Acid
- Magaca Badeecada: Heerka Quudinta Chelate ee Copper Amino Acid
- Muuqaalka: Dhagaxyada cagaaran ee bunniga ah
- Xuduudaha fiisikiska kiimikada
a) Naxaas: ≥ 10.0%
b) Wadarta amino acids-ka: ≥ 20.0%
c) Heerka Chelation-ka: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Rasaasta: ≤ 5 mg/kg
f) Kaadmiyam: ≤ 5 mg/kg
g) Qoyaanka ku jira: ≤ 5.0%
h) Hagaajinta: Dhammaan walxaha waxay maraan 20 shabaq, iyadoo cabbirka walxaha ugu muhiimsan uu yahay 60-80 shabaq
n=0,1,2,... waxay muujinaysaa naxaasta chelated ee dipeptides, tripeptides, iyo tetrapeptides
Diglycerin
Qaab-dhismeedka chelates-ka peptide-ka yar yar
Astaamaha Darajada Quudinta Chelate ee Copper Amino Acid
- Badeecadani waa macdan raad dabiici ah oo dhan oo lagu sameeyay hab gaar ah oo lagu magacaabo chelating oo leh peptides yar yar oo ah molecule enzymatic warshad saafi ah oo ah substrates chelating iyo walxaha raadadka.
- Badeecadani waa mid kiimiko ahaan deggan waxayna si weyn u yareyn kartaa waxyeelada ay u geysaneyso fiitamiinnada iyo dufanka, iwm.
- Isticmaalka badeecadani waxay gacan ka geysaneysaa hagaajinta tayada quudinta. Badeecada waxaa lagu nuugaa marinnada peptide-ka yar yar iyo amino acid-ka, taasoo yaraynaysa tartanka iyo iska soo horjeedka walxaha kale ee raadadka ah, waxayna leedahay heerka nuugista noolaha iyo isticmaalka ugu fiican.
- Naxaasku waa qaybta ugu weyn ee unugyada dhiigga cas, unugyada isku xira, lafaha, oo ku lug leh jirka enzymes kala duwan, waxay xoojisaa shaqada difaaca jirka, saameynta antibiyootigga, waxay kordhin kartaa miisaanka maalinlaha ah, waxay hagaajin kartaa mushaharka quudinta.
Isticmaalka iyo Waxtarka Fasalka Quudinta Chelate ee Naxaasta Amino Acid
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t agab qiimo buuxa leh) | Waxyaabaha ku jira quudinta qiimaha buuxa (mg/kg) | Waxtarka |
| Beer | 400~700 | 60~105 | 1. Hagaajinta waxqabadka taranka iyo isticmaalka sanadaha beerashada; 2. Kordhi firfircoonida uurjiifka iyo doofaarrada; 3. Hagaajinta difaaca jirka iyo iska caabbinta cudurrada. |
| Doofaar | 300~600 | 45~90 | 1. Waxay faa'iido u leedahay hagaajinta shaqooyinka dhiigga iyo difaaca jirka, kor u qaadida iska caabbinta walwalka iyo iska caabbinta cudurrada; 2. Kordhi heerka koritaanka oo si weyn u hagaaji waxtarka quudinta. |
| Doofaar naaxinta | 125 | Janaayo 18.5 | |
| Shimbir | 125 | Janaayo 18.5 | 1. Hagaajinta iska caabbinta walbahaarka iyo yaraynta dhimashada; 2. Hagaajinta magdhawga quudinta iyo kordhinta heerka koritaanka. |
| Xayawaanka biyaha | Kalluun 40～70 | 6~10.5 | 1. Kor u qaad kobaca, hagaaji magdhawga quudinta; 2. Ka hortagga walbahaarka, yaree cudurrada iyo dhimashada. |
| Shrimp 150～200 | 22.5~30 | ||
| Xoolaha xoolaha ah ee aan dhaqin g/madaxa maalinta | Janaayo 0.75 | 1. Ka hortag qallooca kala-goysyada tibia, khalkhalka dhaqdhaqaaqa "gadaal qaloocan", gariir, dhaawaca muruqa wadnaha; 2. Ka hortag timaha ama dahaarka timaha, noqdaan kuwo adag, lumiyaan qalooca caadiga ah, ka hortagga soo ifbaxa "dhibco cawlan" oo ku yaal goobada indhaha; 3. Ka hortagga miisaanka oo yaraada, shubanka, iyo soo saarista caanaha oo yaraada. |
2. Heerka quudinta Ferrous Amino Acid Chelate
- Magaca Badeecada: Ferrous Amino Acid Chelate Feed Fasalka Quudinta
- Muuqaalka: Dhagaxyada cagaaran ee bunniga ah
- Xuduudaha fiisikiska kiimikada
a) Bir: ≥ 10.0%
b) Wadarta amino acids-ka: ≥ 19.0%
c) Heerka Chelation-ka: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Rasaasta: ≤ 5 mg/kg
f) Kaadmiyam: ≤ 5 mg/kg
g) Qoyaanka ku jira: ≤ 5.0%
h) Hagaajinta: Dhammaan walxaha waxay maraan 20 shabaq, iyadoo cabbirka walxaha ugu muhiimsan uu yahay 60-80 shabaq
n=0,1,2,... waxay tilmaamaysaa zinc chelated ah oo loogu talagalay dipeptides, tripeptides, iyo tetrapeptides
Astaamaha Heerka Feed Chelate ee Ferrous Amino Acid
- Badeecadani waa macdan raad dabiici ah oo lagu sameeyay hab gaar ah oo lagu magacaabo chelating oo leh peptides yar yar oo ah molecule enzymatic molecule oo saafi ah sida substrates chelating iyo curiyeyaasha raadadka;
- Badeecadani waa mid kiimiko ahaan deggan waxayna si weyn u yareyn kartaa waxyeelada ay u geysaneyso fiitamiinnada iyo dufanka, iwm. Isticmaalka badeecadani waxay gacan ka geysaneysaa hagaajinta tayada quudinta;
- Badeecada waxaa lagu nuugaa marinnada peptide-ka yar yar iyo amino acid-ka, taasoo yaraynaysa tartanka iyo iska soo horjeedka walxaha kale ee raadadka ah, waxayna leedahay heerka nuugista noolaha iyo isticmaalka ugu fiican;
- Badeecadani waxay ka gudbi kartaa caqabadda mandheerta iyo qanjirka naasaha, waxay ka dhigi kartaa uurjiifka mid caafimaad qaba, waxay kordhin kartaa miisaanka dhalashada iyo miisaanka naas-nuujinta, waxayna yareyn kartaa heerka dhimashada; Birtu waa qayb muhiim ah oo ka mid ah hemoglobin iyo myoglobin, taas oo si wax ku ool ah uga hortagi karta dhiig-yaraanta birta iyo dhibaatooyinkeeda.
Isticmaalka iyo Waxtarka Heerka Feed Chelate ee Ferrous Amino Acid
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t agab qiimo buuxa leh) | Waxyaabaha ku jira quudinta qiimaha buuxa (mg/kg) | Waxtarka |
| Beer | 300~800 | 45~120 | 1. Hagaajinta waxqabadka taranka iyo cimriga isticmaalka ee abuurka; 2. hagaajinta miisaanka dhalashada, miisaanka naas-nuujinta iyo isku-midnimada doofaarka si loo helo waxqabad wax soo saar oo wanaagsan xilliga dambe; 3. Hagaajinta kaydinta birta ee doofaarrada nuugaya iyo xoojinta birta ee caanaha si looga hortago dhiig-yarida birta ee doofaarrada nuugaya. |
| Doofaar iyo doofaarro naaxinaya | Doofaar 300～600 | 45~90 | 1. Hagaajinta difaaca jirka ee doofaarrada, kor u qaadista iska caabbinta cudurka iyo hagaajinta heerka badbaadada; 2. Kordhi heerka koritaanka, hagaaji beddelka quudinta, kordhi miisaanka naas-nuujinta iyo isku-midnimada, iyo yaraynta dhacdooyinka doofaarrada cudurrada; 3. Hagaajinta heerka myoglobin iyo myoglobin, ka hortagga iyo daaweynta dhiig-yaraanta birta la'aanta ah, ka dhig maqaarka doofaarka mid casaan ah oo si cad u hagaajiya midabka hilibka. |
| Doofaar naaxinta 200~400 | 30~60 | ||
| Shimbir | 300~400 | 45~60 | 1. Hagaajinta beddelka quudinta, kordhi heerka koritaanka, hagaajinta awoodda ka hortagga walbahaarka iyo yaraynta dhimashada; 2. Hagaaji heerka ukunta la dhigo, yaree heerka ukunta la jabiyay oo sii qoto dheeree midabka jaallaha; 3. Hagaaji heerka bacriminta iyo heerka ukunta taranka iyo heerka badbaadada ee digaagga yaryar. |
| Xayawaanka biyaha | 200~300 | 30~45 | 1. Kor u qaad kobaca, hagaaji beddelka quudinta; 2. Hagaajinta xakamaynta walbahaarka, yaree cudurrada iyo dhimashada. |
3. Heerka Quudinta Zinc Amino Acid Chelate
- Magaca Badeecada: Heerka Quudinta Chelate ee Zinc Amino Acid
- Muuqaal: granules bunni-jaalle ah
- Xuduudaha fiisikiska kiimikada
a) Zinc: ≥ 10.0%
b) Wadarta amino acids-ka: ≥ 20.5%
c) Heerka Chelation-ka: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Rasaasta: ≤ 5 mg/kg
f) Kaadmiyam: ≤ 5 mg/kg
g) Qoyaanka ku jira: ≤ 5.0%
h) Hagaajinta: Dhammaan walxaha waxay maraan 20 shabaq, iyadoo cabbirka walxaha ugu muhiimsan uu yahay 60-80 shabaq
n=0,1,2,... waxay tilmaamaysaa zinc chelated ah oo loogu talagalay dipeptides, tripeptides, iyo tetrapeptides
Astaamaha Heerka Feed Chelate ee Zinc Amino Acid
Badeecadani waa macdan raad ah oo dabiici ah oo lagu sameeyo hab gaar ah oo lagu sameeyo chelating oo leh peptides yar yar oo ah molecule enzymatic warshad saafi ah oo ah substrates chelating iyo walxaha raad raaca;
Badeecadani waa mid kiimiko ahaan deggan waxayna si weyn u yareyn kartaa waxyeelada ay u geysaneyso fiitamiinnada iyo dufanka, iwm.
Isticmaalka badeecadani waxay gacan ka geysaneysaa hagaajinta tayada quudinta; Badeecada waxaa lagu nuugaa marinnada peptide-ka yar yar iyo amino acid-ka, taasoo yaraynaysa tartanka iyo iska soo horjeedka walxaha kale ee raadadka ah, waxayna leedahay heerka nuugista noolaha iyo isticmaalka ugu fiican;
Badeecadani waxay hagaajin kartaa difaaca jirka, kor u qaadi kartaa koritaanka, kordhin kartaa beddelka quudinta waxayna hagaajin kartaa dhalaalka dhogorta;
Zinc waa qayb muhiim ah oo ka kooban in ka badan 200 oo ensaym, unugyada epithelial, ribose iyo gustatin. Waxay kor u qaaddaa faafitaanka degdega ah ee unugyada dhadhanka ee xuubka carrabka waxayna nidaamisaa rabitaanka cuntada; waxay joojisaa bakteeriyada waxyeelada leh ee xiidmaha; waxayna leedahay shaqada antibiyootigyada, taas oo hagaajin karta shaqada dheecaanka ee habdhiska dheefshiidka iyo dhaqdhaqaaqa ensaymyada ee unugyada iyo unugyada.
Isticmaalka iyo Waxtarka Fasalka Quudinta Chelate ee Zinc Amino Acid
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t agab qiimo buuxa leh) | Waxyaabaha ku jira quudinta qiimaha buuxa (mg/kg) | Waxtarka |
| Beerta uurka leh iyo kuwa nuujinaya | 300~500 | 45～75 | 1. Hagaajinta waxqabadka taranka iyo cimriga isticmaalka ee abuurka; 2. Hagaajinta firfircoonida uurjiifka iyo doofaarka, kor u qaadida iska caabbinta cudurrada, iyo ka dhig inay yeeshaan waxqabad wax soo saar oo wanaagsan marxaladda dambe; 3. Hagaaji xaaladda jireed ee abuurka uurka leh iyo miisaanka dhalashada ee eeyaha doofaarka. |
| Doofaar nuugaya, doofaarro iyo doofaarro cayilinaya | 250~400 | 37.5~60 | 1. Hagaajinta difaaca jirka ee doofaarrada, yaraynta shubanka iyo dhimashada; 2. Hagaajinta dhadhanka cuntada, kordhinta qaadashada quudinta, kordhinta heerka koritaanka iyo hagaajinta beddelka quudinta; 3. Ka dhig jaakadda doofaarka mid dhalaalaya oo hagaaji tayada hilibka iyo tayada hilibka. |
| Shimbir | 300~400 | 45~60 | 1. Hagaaji dhalaalka baalasha; 2. Hagaajinta heerka dhalmada, heerka bacriminta iyo heerka dillaaca ukunta taranka, iyo xoojinta awoodda midabaynta ee jaallaha ukunta; 3. Hagaajinta awoodda ka hortagga walbahaarka iyo yaraynta dhimashada; 4. Hagaaji beddelka quudinta oo kordhi heerka koritaanka. |
| Xayawaanka biyaha | Janaayo 300 | 45 | 1. Kor u qaad kobaca, hagaaji beddelka quudinta; 2. Hagaajinta xakamaynta walbahaarka, yaree cudurrada iyo dhimashada. |
| Xoolaha xoolaha ah ee aan dhaqin g/madaxa maalinta | 2.4 | 1. Hagaajinta wax soo saarka caanaha, ka hortagga naas-nuujinta iyo qudhunka, iyo yaraynta unugyada somatic ee ku jira caanaha; 2. Kor u qaad koritaanka, hagaaji beddelka quudinta iyo hagaajinta tayada hilibka. |
4. Heerka quudinta Chelate-ka ee Manganese Amino Acid
- Magaca Badeecada: Manganese Amino Acid Chelate Feed Fasalka Quudinta Chelate
- Muuqaal: granules bunni-jaalle ah
- Xuduudaha fiisikiska kiimikada
a) Mn: ≥ 10.0%
b) Wadarta amino acids-ka: ≥ 19.5%
c) Heerka Chelation-ka: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Rasaasta: ≤ 5 mg/kg
f) Kaadmiyam: ≤ 5 mg/kg
g) Qoyaanka ku jira: ≤ 5.0%
h) Hagaajinta: Dhammaan walxaha waxay maraan 20 shabaq, iyadoo cabbirka walxaha ugu muhiimsan uu yahay 60-80 shabaq
n=0, 1,2,... waxay tilmaamaysaa manganese chelated ee dipeptides, tripeptides, iyo tetrapeptides
Astaamaha Heerka Quudinta Chelate ee Manganese Amino Acid
Badeecadani waa macdan raad ah oo dabiici ah oo lagu sameeyo hab gaar ah oo lagu sameeyo chelating oo leh peptides yar yar oo ah molecule enzymatic warshad saafi ah oo ah substrates chelating iyo walxaha raad raaca;
Badeecadani waa mid kiimiko ahaan deggan waxayna si weyn u yareyn kartaa waxyeelada ay u geysaneyso fiitamiinnada iyo dufanka, iwm. Isticmaalka badeecadani waxay gacan ka geysaneysaa hagaajinta tayada quudinta;
Badeecada waxaa lagu nuugaa marinnada peptide-ka yar yar iyo amino acid-ka, taasoo yaraynaysa tartanka iyo iska soo horjeedka walxaha kale ee raadadka ah, waxayna leedahay heerka nuugista noolaha iyo isticmaalka ugu fiican;
Badeecadu waxay si weyn u wanaajin kartaa heerka koritaanka, waxay hagaajin kartaa beddelka quudinta iyo xaaladda caafimaad; waxayna si cad u wanaajin kartaa heerka dhalmada, heerka dhalmada iyo heerka caafimaadka leh ee digaagga taranka.
Manganese waa lagama maarmaan u ah koritaanka lafaha iyo dayactirka unugyada isku xiran. Waxay si dhow ula xiriirtaa ensaymo badan; waxayna ka qayb qaadataa dheef-shiid kiimikaadka karbohaydraytka, dufanka iyo borotiinka, taranka iyo jawaab celinta difaaca jirka.
Isticmaalka iyo Waxtarka Heerka Quudinta Chelate ee Manganese Amino Acid
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t agab qiimo buuxa leh) | Waxyaabaha ku jira quudinta qiimaha buuxa (mg/kg) | Waxtarka |
| Doofaar tarmo | 200~300 | 30~45 | 1. Kor u qaadida horumarka caadiga ah ee xubnaha galmada iyo hagaajinta dhaqdhaqaaqa shahwada; 2. Hagaaji awoodda taranka ee doofaarrada taranka iyo yaraynta caqabadaha taranka. |
| Doofaar iyo doofaarro naaxinaya | 100~250 | 15~37.5 | 1. Waa faa'iido in la hagaajiyo hawlaha difaaca jirka, lana hagaajiyo awoodda ka hortagga walbahaarka iyo iska caabbinta cudurrada; 2. Kor u qaad koritaanka oo si weyn u hagaaji beddelka quudinta; 3. Hagaaji midabka iyo tayada hilibka, oo hagaaji boqolkiiba hilibka caatada ah. |
| Shimbir | 250~350 | 37.5~52.5 | 1. Hagaajinta awoodda ka hortagga walbahaarka iyo yaraynta dhimashada; 2. Hagaajinta heerka dhalmada, heerka bacriminta iyo heerka dillaaca ukunta taranka, hagaajinta tayada qolofka ukunta iyo yaraynta heerka jabka qolofka; 3. Kor u qaadida koritaanka lafaha iyo yaraynta cudurrada lugaha. |
| Xayawaanka biyaha | 100~200 | 15~30 | 1. Kor u qaad koritaanka oo hagaaji awooddiisa ka hortagga walbahaarka iyo iska caabbinta cudurrada; 2. Hagaaji dhaqdhaqaaqa shahwada iyo heerka dillaaca ukunta la bacrimiyey. |
| Xoolaha xoolaha ah ee aan dhaqin g/madaxa maalinta | Lo'da 1.25 | 1. Ka hortag khalkhalka isku-darka aashitada dufanka leh iyo dhaawaca unugyada lafaha; 2. Hagaajinta awoodda taranka, ka hortagga ilmo soo rididda iyo curyaaminta dhalmada kadib ee xayawaanka dheddigga ah, yaraynta dhimashada weylaha iyo baraarka, iyo kordhinta miisaanka dhallaanka ee xayawaanka yaryar. | |
| Ri 0.25 |
Qaybta 6aad FAB ee Chelate-yada Peptide-ka yar yar
| S/N | F: Sifooyinka Shaqada | A: Kala duwanaanshaha tartanka | B: Faa'iidooyinka ay keenaan kala duwanaanshaha tartanka ee isticmaalayaasha |
| 1 | Xakamaynta xulashada alaabta ceeriin ah | Xullo hydrolysis-ka enzymatic-ka dhirta saafiga ah ee peptides-ka yaryar | Badbaado bayooloji oo sareysa, iska ilaalinta cunista dadka |
| 2 | Tiknoolajiyada dheefshiidka jihada ee ensaymka bayoolojiga ee borotiinka labanlaab ah | Saamiga sare ee peptides-ka yaryar ee molecular-ka | "Bartilmaameedyo" badan, kuwaas oo aan si fudud loo dhergin karin, oo leh dhaqdhaqaaq bayooloji oo sarreeya iyo xasillooni wanaagsan |
| 3 | Tiknoolajiyadda buufinta cadaadiska iyo qalajinta ee horumarsan | Badeeco granular ah, oo leh cabbir isku mid ah oo walxaha ah, dareere wanaagsan, oo aan si fudud loo nuugin qoyaanka | Hubi in si fudud loo isticmaali karo, isku-darka isku midka ah ee quudinta oo dhammaystiran |
| Biyo yar (≤ 5%), taas oo si weyn u yareysa saameynta ay keenaan fiitamiinnada iyo diyaarinta ensaymyada | Hagaaji xasilloonida alaabta quudinta | ||
| 4 | Tiknoolajiyada xakamaynta wax soo saarka ee horumarsan | Geedi socodka gebi ahaanba xiran, heer sare oo kontorool otomaatig ah | Tayada amniga iyo xasilloonida leh |
| 5 | Tiknoolajiyada xakamaynta tayada sare leh | Samee oo hagaaji hababka falanqaynta sayniska iyo kuwa horumarsan iyo hababka xakamaynta ee lagu ogaanayo arrimaha saameeya tayada badeecada, sida borotiinka aashitada ku milma, qaybinta miisaanka molecular, amino acids iyo heerka chelating | Hubi tayada, hubi waxtarka iyo hagaajinta hufnaanta |
Qaybta 7aad Isbarbardhigga Tartannada
Heerka Caadiga ah ee VS Heerka Caadiga ah
Isbarbardhigga qaybinta peptide iyo heerka chelation ee alaabada
| Badeecadaha Sustar | Saamiga peptides-ka yaryar(180-500) | Badeecadaha Zinpro | Saamiga peptides-ka yaryar(180-500) |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | HELITAANKA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Badeecadaha Sustar | Heerka Chelation-ka | Badeecadaha Zinpro | Heerka Chelation-ka |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | HELITAANKA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
Saamiga peptides-ka yaryar ee Sustar ayaa wax yar ka hooseeya kan Zinpro, heerka chelation-ka ee alaabada Sustar-na wax yar ayuu ka sarreeyaa kan alaabada Zinpro.
Isbarbardhigga waxa ku jira 17 amino acids ah oo ku jira badeecooyin kala duwan
| Magaca amino acids | Naxaasta Sustar Amino Acid Chelate Heerka Quudinta | Zinpro's HELID naxaas | Sustar's Ferrous Amino Acid C Quudinta helate Fasal | Zinpro's ayaa diyaar ah bir | Manganese-ka Sustar Amino Acid Chelate Heerka Quudinta | Zinpro's ayaa diyaar ah manganese | Sink Sustar Asiidh Amino ah Fasalka Quudinta Chelate | Zinpro's ayaa diyaar ah zinc |
| aashitada aspartic (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycin (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanin (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Faalin (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Janaayo 0.75 | 0.44 |
| Fenylalanin (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Borotiin (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Wadarta amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Guud ahaan, saamiga amino acids-ka ee ku jira badeecadaha Sustar ayaa ka sarreeya kan ku jira badeecadaha Zinpro.
Qaybta 8aad Saamaynta isticmaalka
Saamaynta ilo kala duwan oo macdan ah oo raad ah ay ku leeyihiin waxqabadka wax soo saarka iyo tayada ukunta ee digaagga dhigista xilliga dambe ee dhalmada
Habka Wax Soo Saarka
- Tiknoolajiyada chelation ee bartilmaameedsan
- Tiknoolajiyada xiirashada ee emulsification
- Tiknoolajiyada buufinta cadaadiska iyo qalajinta
- Tiknoolajiyada qaboojiyaha iyo ka saarista qoyaanka
- Tiknoolajiyadda xakamaynta deegaanka ee horumarsan
Lifaaqa A: Hababka loo Go'aamiyo qaybinta tirada molecular ee qaraabada ah ee peptides
Qaadashada heerka: GB/T 22492-2008
1 Mabda'a Tijaabada:
Waxaa lagu go'aamiyay koromatografiga sifeynta jelka ee waxqabadka sare leh. Taasi waa, iyadoo la adeegsanayo buuxinta daloolsan marxalad taagan, iyadoo lagu saleynayo farqiga cabbirka cufka molecular ee qaraabada ah ee qaybaha muunadda ee kala-soocidda, oo lagu ogaado isku-xidhka peptide ee hirarka nuugista ultraviolet-ka ee 220nm, iyadoo la adeegsanayo software-ka habaynta xogta ee loogu talagalay go'aaminta qaybinta cufka molecular ee qaraabada ah iyadoo la adeegsanayo koromatografiga sifeynta jelka (tusaale ahaan, software-ka GPC), koromatografiyada iyo xogtooda ayaa la farsameeyay, oo loo xisaabiyay si loo helo cabbirka cufka molecular ee qaraabada ah ee peptide-ka soybean-ka iyo baaxadda qaybinta.
2. Reagents
Biyaha tijaabada ah waa inay buuxiyaan shuruudaha biyaha labaad ee GB/T6682, isticmaalka walxaha firfircoon, marka laga reebo qodobbada gaarka ah, waa kuwo si falanqayn ah u saafi ah.
2.1 Waxyaabaha fal-celinta sameeya waxaa ka mid ah acetonitrile (oo ah mid saafi ah oo chromatographic ah), trifluoroacetic acid (oo ah mid saafi ah oo chromatographic ah),
2.2 Walxaha caadiga ah ee loo isticmaalo qalooca cabbiraadda qaybinta cufnaanta molecular ee qaraabada ah: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Qalabka iyo qalabka
3.1 Chromatograph-ka Dareeraha ee Waxqabadka Sare (HPLC): waa goob shaqo oo koromografi ah ama isku-dhafan oo leh qalabka ogaanshaha UV iyo barnaamijka habaynta xogta GPC.
3.2 Qalabka shaandhaynta faakuumka ee wejiga wareega iyo nadiifinta gaaska.
3.3 Dheelitirka elektaroonigga ah: qiimaha qalin-jabinta 0.000 1g.
4 Tallaabo oo Hawlgal ah
4.1 Xaaladaha koromogaraafka iyo tijaabooyinka la qabsiga nidaamka (xaaladaha tixraaca)
4.1.1 Tiirka Chromatografiga: TSKgelG2000swxl300 mm × 7.8 mm (dhexroor gudaha ah) ama tiirar kale oo jel ah oo isku nooc ah oo leh waxqabad la mid ah oo ku habboon go'aaminta borotiinnada iyo peptides-ka.
4.1.2 Marxaladda guurguurta: Acetonitrile + biyo + aashitada trifluoroacetic = 20 + 80 + 0.1.
4.1.3 Mowjadda ogaanshaha: 220 nm.
4.1.4 Heerka socodka: 0.5 mL/daqiiqo.
4.1.5 Waqtiga ogaanshaha: 30 daqiiqo.
4.1.6 Mugga muunadda duritaanka: 20μL.
4.1.7 Heerkulka tiirka: heerkulka qolka.
4.1.8 Si nidaamka koromografiga looga dhigo mid buuxiya shuruudaha ogaanshaha, waxaa la dejiyay in xaaladaha koromografiga ee kor ku xusan, hufnaanta tiirka jel chromatographic, tusaale ahaan, tirada tiyoolajiyadda ee taarikada (N), aysan ka yarayn 10000 oo lagu xisaabiyay iyadoo lagu saleynayo meelaha ugu sarreeya ee heerka tripeptide (Glycine-Glycine-Glycine).
4.2 Soo saarista qaloocyada heerka cufka molecular-ka ee qaraabada ah
Xalalka heerka peptide-ka ee molecular-ka ee kala duwan ee kor ku xusan oo leh tiro badan oo ah 1 mg / mL ayaa lagu diyaariyey isbarbardhigga marxaladda guurguurta, oo lagu qasay saami gaar ah, ka dibna lagu sifeeyay xuub marxalad dabiici ah oo leh cabbirka daloolka oo ah 0.2 μm ~ 0.5 μm waxaana lagu duray muunadda, ka dibna chromatogram-yada heerarka ayaa la helay. Qalloocyada cabbiraadda cufka molecular-ka ee qaraabada ah iyo isle'egyada ay sameeyeen waxaa lagu helay iyadoo la sawirayo logarithm-ka cufka molecular-ka ee qaraabada ah iyadoo laga soo horjeedo waqtiga haynta ama dib-u-noqoshada toosan.
4.3 Daaweynta muunadda
Si sax ah u miisaan 10mg oo muunad ah oo ku jirta dhalo muunad 10mL ah, ku dar marxalad yar oo dhaqaaq ah, oo gariir ultrasonic ah muddo 10 daqiiqo ah, si muunaddu si buuxda u milmiso oo isku qasto, loogu qaso marxaladda dhaqaaqa ilaa miisaanka, ka dibna lagu sifeeyo xuub wejiga dabiiciga ah oo leh cabbir dalool ah oo ah 0.2μm ~ 0.5μm, shaandhadana waxaa lagu falanqeeyay iyadoo loo eegayo xaaladaha koromografiga ee A.4.1.
5. Xisaabinta qaybinta cufnaanta molecular-ka ee qaraabada ah
Ka dib marka la falanqeeyo xalka muunadda ee lagu diyaariyey 4.3 iyadoo la raacayo xaaladaha koromografiga ee 4.1, cufnaanta molecular ee qaraabada ah ee muunadda iyo baaxadda qaybinta waxaa lagu heli karaa iyadoo xogta koromografiga ee muunadda lagu beddelayo qalooca cabbiraadda 4.2 iyadoo la adeegsanayo barnaamijka habaynta xogta GPC. Qaybinta cufnaanta molecular ee qaraabada ah ee peptides-ka kala duwan waxaa lagu xisaabin karaa habka caadiga ah ee aagga ugu sarreeya, sida waafaqsan qaacidada: X=A/A wadarta × 100
Qaaciddada: X - Jajab cufnaanta ee peptide cufnaanta molecular ee wadarta peptide ee muunadda, %;
A - Aagga ugu sarreeya ee peptide cufnaanta molecular ee qaraabada ah;
Wadarta A - wadarta meelaha ugu sarreeya ee peptide kasta oo cufnaanta molecular ah, oo loo xisaabiyay hal meel jajab tobanle ah.
6 Ku celcelin
Farqiga buuxa ee u dhexeeya laba go'aan oo madax-bannaan oo lagu helo xaaladaha ku celcelinta waa inaanu dhaafin 15% celceliska xisaabta ee labada go'aan.
Lifaaqa B: Hababka lagu Go'aaminayo Asiidhyada Bilaashka ah
Ansixinta heerka: Q/320205 KAVN05-2016
1.2 Reagents iyo agabka
Aashitada acetic-ga ee Glacial: si falanqayn ah oo saafi ah
Aashitada Perchloric: 0.0500 mol/L
Tilmaame: 0.1% tilmaame buluug ah oo crystal ah (asiidh glacial acetic ah)
2. Go'aaminta amino acids-ka bilaashka ah
Shaybaarrada waxaa lagu qalajiyey heerkul ah 80°C muddo 1 saac ah.
Muunadda ku rid weel qalalan si uu si dabiici ah ugu qaboojiyo heerkulka qolka ama uu u qaboojiyo heerkul la isticmaali karo.
Ku miisaami qiyaastii 0.1 g oo muunad ah (sax ah ilaa 0.001 g) weel qalalan oo koon ah oo 250 mL ah.
Si dhakhso ah ugu gudub tallaabada xigta si aad uga fogaato muunadda inay nuugto qoyaanka deegaanka.
Ku dar 25 mL oo ah glacial acetic acid oo si fiican isku walaaq muddo aan ka badnayn 5 daqiiqo.
Ku dar 2 dhibcood oo tilmaame buluug ah oo kiristaalo ah
Ku titrate xalka titration-ka caadiga ah ee 0.0500 mol / L (±0.001) ee aashitada perchloric ilaa xalka uu ka beddelo guduud ilaa barta ugu dambeysa.
Qor mugga xalka caadiga ah ee la isticmaalay.
Samee tijaabada madhan isla waqtigaas.
3. Xisaabinta iyo natiijooyinka
Tirada amino acid-ka xorta ah ee X ee ku jirta fal-galka waxaa lagu muujiyaa jajab cuf ah (%) waxaana lagu xisaabiyaa iyadoo la raacayo qaacidada: X = C × (V1-V0) × 0.1445/M × 100%, qaacidada tne:
C - Isugeynta xalka aashitada perchloric ee caadiga ah ee moles halkii litir (mol/L)
V1 - Mugga loo isticmaalo titration-ka muunadaha iyadoo la isticmaalayo xalka caadiga ah ee perchloric acid, millilitir ahaan (mL).
Vo - Mugga loo isticmaalo titration madhan oo leh xalka caadiga ah ee perchloric acid, millilitir (mL);
M - Cufka muunadda, garaam ahaan (g).
0.1445: Celceliska cufka amino acids oo u dhiganta 1.00 mL oo ah xalka perchloric acid ee caadiga ah [c (HClO4) = 1.000 mol / L].
Lifaaqa C: Hababka lagu Go'aaminayo Heerka Chelation-ka ee Sustar
Ansixinta heerarka: Q/70920556 71-2024
1. Mabda'a go'aaminta (Fe tusaale ahaan)
Isku-dhafka birta amino acid-ka waxay leeyihiin milmi aad u hooseeya oo ku jira ethanol aan biyo lahayn, ion-yada birta xorta ahna waxay ku milmaan ethanol aan biyo lahayn, farqiga u dhexeeya milmida u dhaxaysa labada ethanol aan biyo lahayn ayaa loo isticmaalay si loo go'aamiyo heerka chelation ee isku-dhafka birta amino acid-ka.
2. Wakiilada iyo Xalalka
Etanol aan biyo lahayn; inta soo hartayna waxay la mid tahay qodobka 4.5.2 ee GB/T 27983-2011.
3. Tallaabooyinka falanqaynta
Samee laba tijaabo oo is barbar socda. Miisaan 0.1g oo muunad ah ku qalaji 103±2℃ muddo 1 saac ah, sax ah 0.0001g, ku dar 100mL oo ethanol aan biyo lahayn si uu u milmo, shaandhee, shaandhee haraaga lagu dhaqo 100mL oo ethanol aan biyo lahayn ugu yaraan saddex jeer, ka dibna haraaga ku wareeji weel koonka ah oo 250mL ah, ku dar 10mL oo xal sulfuric acid ah sida ku xusan qodobka 4.5.3 ee GB/T27983-2011, ka dibna samee tallaabooyinka soo socda sida ku xusan qodobka 4.5.3 "Kuleyl si uu u milmo ka dibna u daa inuu qaboobo" ee GB/T27983-2011. Samee tijaabada madhan isla waqtigaas.
4. Go'aaminta wadarta guud ee birta
4.1 Mabda'a go'aamintu wuxuu la mid yahay qodobka 4.4.1 ee GB/T 21996-2008.
4.2. Wakiilada iyo Xalalka
4.2.1 Asiidh isku dhafan: Ku dar 150mL oo asiidh sulfuric ah iyo 150mL oo fosfoorik ah 700mL oo biyo ah oo si fiican isku walaaq.
4.2.2 Xalka tilmaame ee Sodium diphenylamine sulfonate: 5g/L, oo loo diyaariyey sida waafaqsan GB/T603.
4.2.3 Xalka titration-ka ee heerka Cerium sulfate: fiirsashada c [Ce (SO4) 2] = 0.1 mol/L, oo loo diyaariyey sida waafaqsan GB/T601.
4.3 Tallaabooyinka falanqaynta
Samee laba tijaabo oo is barbar socda. Miisaan 0.1g oo muunad ah, sax ah 020001g, ku rid dhalo koon ah oo 250mL ah, ku dar 10mL oo aashito isku dhafan ah, ka dib marka la milo, ku dar 30ml oo biyo ah iyo 4 dhibcood oo ah xalka tilmaame sodium dianiline sulfonate, ka dibna samee tallaabooyinka soo socda sida ku xusan qodobka 4.4.2 ee GB/T21996-2008. Samee tijaabada madhan isla waqtigaas.
4.4 Matalaadda natiijooyinka
Wadarta guud ee ka kooban birta X1 ee isku-dhafka birta amino acid marka la eego tirada jajabka birta, qiimaha lagu muujiyay %, waxaa lagu xisaabiyay qaacidada (1):
X1=(V-V0)×C×M×10-3×100
Qaaciddada: V - mugga xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka tijaabada, mL;
V0 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka madhan, mL;
C - Fiirsashada dhabta ah ee xalka caadiga ah ee cerium sulfate, mol/L
5. Xisaabinta maadada birta ee chelates
Tirada birta X2 ee ku jirta chelate marka la eego qaybta cufka ee birta, qiimaha lagu muujiyay %, waxaa lagu xisaabiyay qaacidada: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Qaaciddada: V1 - mugga xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka tijaabada, mL;
V2 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka madhan, mL;
C - Fiirsashada dhabta ah ee xalka caadiga ah ee cerium sulfate, mol/L;
0.05585 - cufnaanta birta birta ah ee lagu muujiyey garaamyo u dhigma 1.00 mL oo ah xalka caadiga ah ee cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1- Cufka muunadda, g. Celceliska xisaabta ee natiijooyinka go'aaminta barbar socda u qaado natiijooyinka go'aaminta, farqiga buuxa ee natiijooyinka go'aaminta barbar socdana kama badna 0.3%.
6. Xisaabinta heerka chelation-ka
Heerka Chelation-ka X3, qiimaha lagu muujiyay %, X3 = X2/X1 × 100
Lifaaqa C: Hababka lagu Go'aaminayo Heerka Chelation-ka ee Zinpro
Ansixinta heerka: Q/320205 KAVNO7-2016
1. Waxyaabaha dib-u-warshadaynta iyo agabka
a) Aashitada Glacial acetic: si falanqayn ah oo saafi ah; b) Aashitada Perchloric: 0.0500mol/L; c) Tilmaame: 0.1% tilmaame buluug ah oo crystal ah (aasidhka glacial acetic)
2. Go'aaminta amino acids-ka bilaashka ah
2.1 Shaybaarrada waxaa lagu qalajiyey heerkul ah 80°C muddo 1 saac ah.
2.2 Muunadda ku rid weel qalalan si ay si dabiici ah ugu qaboojiso heerkulka qolka ama ugu qaboojiso heerkul la isticmaali karo.
2.3 Miisaan qiyaastii 0.1 g oo muunad ah (sax ah ilaa 0.001 g) ku rid dhalo qallalan oo koontarool ah oo 250 mL ah
2.4 Si dhakhso ah ugu gudub tallaabada xigta si aad uga fogaato muunadda inay nuugto qoyaanka deegaanka.
2.5 Ku dar 25mL oo ah asiidh glacial acetic ah oo si fiican isku walaaq muddo aan ka badnayn 5 daqiiqo.
2.6 Ku dar 2 dhibcood oo tilmaame buluug ah oo crystal ah.
2.7 Ku shub xal titration caadi ah oo ah 0.0500mol/L (±0.001) oo ah perchloric acid ilaa xalku uu ka beddelo guduud una beddelo cagaar muddo 15 ilbiriqsi ah iyada oo aan la beddelin midabka barta ugu dambeysa.
2.8 Diiwaangeli mugga xalka caadiga ah ee la isticmaalay.
2.9 Samee tijaabada madhan isla waqtigaas.
3. Xisaabinta iyo natiijooyinka
Tirada amino acid-ka xorta ah ee X ee ku jirta fal-galka waxaa lagu muujiyaa jajab cuf ah (%), oo lagu xisaabiyay qaacidada (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
Qaaciddada: C - isku-darka xalka aashitada perchloric ee caadiga ah ee ku jira moles halkii litir (mol/L)
V1 - Mugga loo isticmaalo titration-ka muunadaha iyadoo la isticmaalayo xalka caadiga ah ee perchloric acid, millilitir ahaan (mL).
Vo - Mugga loo isticmaalo titration madhan oo leh xalka caadiga ah ee perchloric acid, millilitir (mL);
M - Cufka muunadda, garaam ahaan (g).
0.1445 - Celceliska cufka amino acids oo u dhiganta 1.00 mL oo ah xalka perchloric acid ee caadiga ah [c (HClO4) = 1.000 mol / L].
4. Xisaabinta heerka chelation-ka
Heerka chelation-ka ee muunadda waxaa lagu muujiyaa jajab cuf (%), oo lagu xisaabiyay qaacidada (2): heerka chelation = (wadarta guud ee amino acid - wadarta amino acid-ka bilaashka ah) / wadarta guud ee amino acid × 100%.
Waqtiga boostada: Sebtembar-17-2025
