2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Walxaha caadiga ah ee loo isticmaalo qalooca cabbiraadda qaybinta cufnaanta molecular ee qaraabada ah: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Qalabka iyo qalabka
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Guud ahaan, saamiga amino acids-ka ee ku jira badeecadaha Sustar ayaa ka sarreeya kan ku jira badeecadaha Zinpro.
Qaybta 8aad Saamaynta isticmaalka
Saamaynta ilo kala duwan oo macdan ah oo raad ah ay ku leeyihiin waxqabadka wax soo saarka iyo tayada ukunta ee digaagga dhigista xilliga dambe ee dhalmada
Habka Wax Soo Saarka
Tiknoolajiyada chelation ee bartilmaameedsan
Tiknoolajiyada xiirashada ee emulsification
Tiknoolajiyada buufinta cadaadiska iyo qalajinta
Tiknoolajiyada qaboojiyaha iyo ka saarista qoyaanka
Tiknoolajiyadda xakamaynta deegaanka ee horumarsan
Lifaaqa A: Hababka loo Go'aamiyo qaybinta tirada molecular ee qaraabada ah ee peptides
Qaadashada heerka: GB/T 22492-2008
1 Mabda'a Tijaabada:
Waxaa lagu go'aamiyay koromatografiga sifeynta jelka ee waxqabadka sare leh. Taasi waa, iyadoo la adeegsanayo buuxinta daloolsan marxalad taagan, iyadoo lagu saleynayo farqiga cabbirka cufka molecular ee qaraabada ah ee qaybaha muunadda ee kala-soocidda, oo lagu ogaado isku-xidhka peptide ee hirarka nuugista ultraviolet-ka ee 220nm, iyadoo la adeegsanayo software-ka habaynta xogta ee loogu talagalay go'aaminta qaybinta cufka molecular ee qaraabada ah iyadoo la adeegsanayo koromatografiga sifeynta jelka (tusaale ahaan, software-ka GPC), koromatografiyada iyo xogtooda ayaa la farsameeyay, oo loo xisaabiyay si loo helo cabbirka cufka molecular ee qaraabada ah ee peptide-ka soybean-ka iyo baaxadda qaybinta.
2. Reagents
Biyaha tijaabada ah waa inay buuxiyaan shuruudaha biyaha labaad ee GB/T6682, isticmaalka walxaha firfircoon, marka laga reebo qodobbada gaarka ah, waa kuwo si falanqayn ah u saafi ah.
2.1 Waxyaabaha fal-celinta sameeya waxaa ka mid ah acetonitrile (oo ah mid saafi ah oo chromatographic ah), trifluoroacetic acid (oo ah mid saafi ah oo chromatographic ah),
2.2 Walxaha caadiga ah ee loo isticmaalo qalooca cabbiraadda qaybinta cufnaanta molecular ee qaraabada ah: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Qalabka iyo qalabka
3.1 Chromatograph-ka Dareeraha ee Waxqabadka Sare (HPLC): waa goob shaqo oo koromografi ah ama isku-dhafan oo leh qalabka ogaanshaha UV iyo barnaamijka habaynta xogta GPC.
3.2 Qalabka shaandhaynta faakuumka ee wejiga wareega iyo nadiifinta gaaska.
3.3 Dheelitirka elektaroonigga ah: qiimaha qalin-jabinta 0.000 1g.
4 Tallaabo oo Hawlgal ah
4.1 Xaaladaha koromogaraafka iyo tijaabooyinka la qabsiga nidaamka (xaaladaha tixraaca)
- 4.1.1 Tiirka Chromatografiga: TSKgelG2000swxl300 mm × 7.8 mm (dhexroor gudaha ah) ama tiirar kale oo jel ah oo isku nooc ah oo leh waxqabad la mid ah oo ku habboon go'aaminta borotiinnada iyo peptides-ka.
- 4.1.2 Marxaladda guurguurta: Acetonitrile + biyo + aashitada trifluoroacetic = 20 + 80 + 0.1.
- 4.1.3 Mowjadda ogaanshaha: 220 nm.
- 4.1.4 Heerka socodka: 0.5 mL/daqiiqo.
- 4.1.5 Waqtiga ogaanshaha: 30 daqiiqo.
- 4.1.6 Mugga muunadda duritaanka: 20μL.
- 4.1.7 Heerkulka tiirka: heerkulka qolka.
- 4.1.8 Si nidaamka koromografiga looga dhigo mid buuxiya shuruudaha ogaanshaha, waxaa la dejiyay in xaaladaha koromografiga ee kor ku xusan, hufnaanta tiirka jel chromatographic, tusaale ahaan, tirada tiyoolajiyadda ee taarikada (N), aysan ka yarayn 10000 oo lagu xisaabiyay iyadoo lagu saleynayo meelaha ugu sarreeya ee heerka tripeptide (Glycine-Glycine-Glycine).
- 4.2 Soo saarista qaloocyada heerka cufka molecular-ka ee qaraabada ah
- Xalalka heerka peptide-ka ee molecular-ka ee kala duwan ee kor ku xusan oo leh tiro badan oo ah 1 mg / mL ayaa lagu diyaariyey isbarbardhigga marxaladda guurguurta, oo lagu qasay saami gaar ah, ka dibna lagu sifeeyay xuub marxalad dabiici ah oo leh cabbirka daloolka oo ah 0.2 μm ~ 0.5 μm waxaana lagu duray muunadda, ka dibna chromatogram-yada heerarka ayaa la helay. Qalloocyada cabbiraadda cufka molecular-ka ee qaraabada ah iyo isle'egyada ay sameeyeen waxaa lagu helay iyadoo la sawirayo logarithm-ka cufka molecular-ka ee qaraabada ah iyadoo laga soo horjeedo waqtiga haynta ama dib-u-noqoshada toosan.
4.3 Daaweynta muunadda
Si sax ah u miisaan 10mg oo muunad ah oo ku jirta dhalo muunad 10mL ah, ku dar marxalad yar oo dhaqaaq ah, oo gariir ultrasonic ah muddo 10 daqiiqo ah, si muunaddu si buuxda u milmiso oo isku qasto, loogu qaso marxaladda dhaqaaqa ilaa miisaanka, ka dibna lagu sifeeyo xuub wejiga dabiiciga ah oo leh cabbir dalool ah oo ah 0.2μm ~ 0.5μm, shaandhadana waxaa lagu falanqeeyay iyadoo loo eegayo xaaladaha koromografiga ee A.4.1.
- 5. Xisaabinta qaybinta cufnaanta molecular-ka ee qaraabada ah
- Ka dib marka la falanqeeyo xalka muunadda ee lagu diyaariyey 4.3 iyadoo la raacayo xaaladaha koromografiga ee 4.1, cufnaanta molecular ee qaraabada ah ee muunadda iyo baaxadda qaybinta waxaa lagu heli karaa iyadoo xogta koromografiga ee muunadda lagu beddelayo qalooca cabbiraadda 4.2 iyadoo la adeegsanayo barnaamijka habaynta xogta GPC. Qaybinta cufnaanta molecular ee qaraabada ah ee peptides-ka kala duwan waxaa lagu xisaabin karaa habka caadiga ah ee aagga ugu sarreeya, sida waafaqsan qaacidada: X=A/A wadarta × 100
- Qaaciddada: X - Jajab cufnaanta ee peptide cufnaanta molecular ee wadarta peptide ee muunadda, %;
- A - Aagga ugu sarreeya ee peptide cufnaanta molecular ee qaraabada ah;
- Wadarta A - wadarta meelaha ugu sarreeya ee peptide kasta oo cufnaanta molecular ah, oo loo xisaabiyay hal meel jajab tobanle ah.
- 6 Ku celcelin
- Farqiga buuxa ee u dhexeeya laba go'aan oo madax-bannaan oo lagu helo xaaladaha ku celcelinta waa inaanu dhaafin 15% celceliska xisaabta ee labada go'aan.
- Lifaaqa B: Hababka lagu Go'aaminayo Asiidhyada Bilaashka ah
- Ansixinta heerka: Q/320205 KAVN05-2016
- 1.2 Reagents iyo agabka
- Aashitada acetic-ga ee Glacial: si falanqayn ah oo saafi ah
- Aashitada Perchloric: 0.0500 mol/L
- Tilmaame: 0.1% tilmaame buluug ah oo crystal ah (asiidh glacial acetic ah)
- 2. Go'aaminta amino acids-ka bilaashka ah
Shaybaarrada waxaa lagu qalajiyey heerkul ah 80°C muddo 1 saac ah.
Muunadda ku rid weel qalalan si uu si dabiici ah ugu qaboojiyo heerkulka qolka ama uu u qaboojiyo heerkul la isticmaali karo.Ku miisaami qiyaastii 0.1 g oo muunad ah (sax ah ilaa 0.001 g) weel qalalan oo koon ah oo 250 mL ah.Si dhakhso ah ugu gudub tallaabada xigta si aad uga fogaato muunadda inay nuugto qoyaanka deegaanka.Ku dar 25 mL oo ah glacial acetic acid oo si fiican isku walaaq muddo aan ka badnayn 5 daqiiqo.Ku dar 2 dhibcood oo tilmaame buluug ah oo kiristaalo ahKu titrate xalka titration-ka caadiga ah ee 0.0500 mol / L (±0.001) ee aashitada perchloric ilaa xalka uu ka beddelo guduud ilaa barta ugu dambeysa.
Qor mugga xalka caadiga ah ee la isticmaalay.
- Samee tijaabada madhan isla waqtigaas.
- 3. Xisaabinta iyo natiijooyinka
- Tirada amino acid-ka xorta ah ee X ee ku jirta fal-galka waxaa lagu muujiyaa jajab cuf ah (%) waxaana lagu xisaabiyaa iyadoo la raacayo qaacidada: X = C × (V1-V0) × 0.1445/M × 100%, qaacidada tne:
- C - Isugeynta xalka aashitada perchloric ee caadiga ah ee moles halkii litir (mol/L)
- V1 - Mugga loo isticmaalo titration-ka muunadaha iyadoo la isticmaalayo xalka caadiga ah ee perchloric acid, millilitir ahaan (mL).
- Vo - Mugga loo isticmaalo titration madhan oo leh xalka caadiga ah ee perchloric acid, millilitir (mL);
M - Cufka muunadda, garaam ahaan (g).
| 0.1445: Celceliska cufka amino acids oo u dhiganta 1.00 mL oo ah xalka perchloric acid ee caadiga ah [c (HClO4) = 1.000 mol / L]. | 4.2.3 Xalka titration-ka ee heerka Cerium sulfate: fiirsashada c [Ce (SO4) 2] = 0.1 mol/L, oo loo diyaariyey sida waafaqsan GB/T601. | |
| Ansixinta heerarka: Q/70920556 71-2024 | 1. Mabda'a go'aaminta (Fe tusaale ahaan) | Isku-dhafka birta amino acid-ka waxay leeyihiin milmi aad u hooseeya oo ku jira ethanol aan biyo lahayn, ion-yada birta xorta ahna waxay ku milmaan ethanol aan biyo lahayn, farqiga u dhexeeya milmida u dhaxaysa labada ethanol aan biyo lahayn ayaa loo isticmaalay si loo go'aamiyo heerka chelation ee isku-dhafka birta amino acid-ka. |
| Qaaciddada: V1 - mugga xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka tijaabada, mL; | Etanol aan biyo lahayn; inta soo hartayna waxay la mid tahay qodobka 4.5.2 ee GB/T 27983-2011. | 3. Tallaabooyinka falanqaynta |
| Samee laba tijaabo oo is barbar socda. Miisaan 0.1g oo muunad ah ku qalaji 103±2℃ muddo 1 saac ah, sax ah 0.0001g, ku dar 100mL oo ethanol aan biyo lahayn si uu u milmo, shaandhee, shaandhee haraaga lagu dhaqo 100mL oo ethanol aan biyo lahayn ugu yaraan saddex jeer, ka dibna haraaga ku wareeji weel koonka ah oo 250mL ah, ku dar 10mL oo xal sulfuric acid ah sida ku xusan qodobka 4.5.3 ee GB/T27983-2011, ka dibna samee tallaabooyinka soo socda sida ku xusan qodobka 4.5.3 "Kuleyl si uu u milmo ka dibna u daa inuu qaboobo" ee GB/T27983-2011. Samee tijaabada madhan isla waqtigaas. | 4. Go'aaminta wadarta guud ee birta | 4.1 Mabda'a go'aamintu wuxuu la mid yahay qodobka 4.4.1 ee GB/T 21996-2008. |
4.2. Wakiilada iyo Xalalka
| 4.2.1 Asiidh isku dhafan: Ku dar 150mL oo asiidh sulfuric ah iyo 150mL oo asiidh fosfoorik ah 700mL oo biyo ah oo si fiican isku walaaq. | 4.2.2 Xalka tilmaame ee Sodium diphenylamine sulfonate: 5g/L, oo loo diyaariyey sida waafaqsan GB/T603. | 4.2.3 Xalka titration-ka ee heerka Cerium sulfate: fiirsashada c [Ce (SO4) 2] = 0.1 mol/L, oo loo diyaariyey sida waafaqsan GB/T601. | |
| 4.3 Tallaabooyinka falanqaynta | Samee laba tijaabo oo is barbar socda. Miisaan 0.1g oo muunad ah, sax ah 020001g, ku rid dhalo koon ah oo 250mL ah, ku dar 10mL oo aashito isku dhafan ah, ka dib marka la milo, ku dar 30ml oo biyo ah iyo 4 dhibcood oo ah xalka tilmaame sodium dianiline sulfonate, ka dibna samee tallaabooyinka soo socda sida ku xusan qodobka 4.4.2 ee GB/T21996-2008. Samee tijaabada madhan isla waqtigaas. | 4.4 Matalaadda natiijooyinka | Wadarta guud ee ka kooban birta X1 ee isku-dhafka birta amino acid marka la eego tirada jajabka birta, qiimaha lagu muujiyay %, waxaa lagu xisaabiyay qaacidada (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka madhan, mL; | V0 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka madhan, mL; | C - Fiirsashada dhabta ah ee xalka caadiga ah ee cerium sulfate, mol/L5. Xisaabinta maadada birta ee chelatesTirada birta X2 ee ku jirta chelate marka la eego qaybta cufka ee birta, qiimaha lagu muujiyay %, waxaa lagu xisaabiyay qaacidada: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Qaaciddada: V1 - mugga xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka tijaabada, mL; | V2 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration ee xalka madhan, mL;nom1- Cufka muunadda, g. Celceliska xisaabta ee natiijooyinka go'aaminta barbar socda u qaado natiijooyinka go'aaminta, farqiga buuxa ee natiijooyinka go'aaminta barbar socdana kama badna 0.3%. | 0.05585 - cufnaanta birta birta ah ee lagu muujiyey garaam u dhigma 1.00 mL oo ah xalka caadiga ah ee cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1- Cufka muunadda, g. Celceliska xisaabta ee natiijooyinka go'aaminta barbar socda u qaado natiijooyinka go'aaminta, farqiga buuxa ee natiijooyinka go'aaminta barbar socdana kama badna 0.3%. | 6. Xisaabinta heerka chelation-kaHeerka Chelation-ka X3, qiimaha lagu muujiyay %, X3 = X2/X1 × 100Lifaaqa C: Hababka lagu Go'aaminayo Heerka Chelation-ka ee Zinpro |
Ansixinta heerka: Q/320205 KAVNO7-2016
1. Waxyaabaha dib-u-warshadaynta iyo agabka
a) Aashitada Glacial acetic: si falanqayn ah oo saafi ah; b) Aashitada Perchloric: 0.0500mol/L; c) Tilmaame: 0.1% tilmaame buluug ah oo crystal ah (aasidhka glacial acetic)
2. Go'aaminta amino acids-ka bilaashka ah
2.1 Shaybaarrada waxaa lagu qalajiyey heerkul ah 80°C muddo 1 saac ah.
2.2 Muunadda ku rid weel qalalan si ay si dabiici ah ugu qaboojiso heerkulka qolka ama ugu qaboojiso heerkul la isticmaali karo.
2.3 Miisaan qiyaastii 0.1 g oo muunad ah (sax ah ilaa 0.001 g) ku rid dhalo qallalan oo koontarool ah oo 250 mL ah
2.4 Si dhakhso ah ugu gudub tallaabada xigta si aad uga fogaato muunadda inay nuugto qoyaanka deegaanka.
2.5 Ku dar 25mL oo ah asiidh glacial acetic ah oo si fiican isku walaaq muddo aan ka badnayn 5 daqiiqo.
2.6 Ku dar 2 dhibcood oo tilmaame buluug ah oo crystal ah.
2.7 Ku shub xal titration caadi ah oo ah 0.0500mol/L (±0.001) oo ah perchloric acid ilaa xalku uu ka beddelo guduud una beddelo cagaar muddo 15 ilbiriqsi ah iyada oo aan la beddelin midabka barta ugu dambeysa.
2.8 Diiwaangeli mugga xalka caadiga ah ee la isticmaalay.
2.9 Samee tijaabada madhan isla waqtigaas.
- 3. Xisaabinta iyo natiijooyinka
- Catalan
- Physicochemical parameters
V1 - Mugga loo isticmaalo titration-ka muunadaha iyadoo la isticmaalayo xalka caadiga ah ee perchloric acid, millilitir ahaan (mL).
Vo - Mugga loo isticmaalo titration madhan oo leh xalka caadiga ah ee perchloric acid, millilitir (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Cinwaanka: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Gobolka Sichuan, Shiinaha
Telefoon: 86-18880477902
Badeecadaha
Macdanta raad aan dabiici ahayn
- Macdanta raadadka dabiiciga ah
- Sawaaxili
- Adeeg gaar ah
- Xiriirin degdeg ah
Astaamaha Shirkadda
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Guji si aad u weydiiso | © Xuquuqda daabacaadda - 2010-2025: Dhammaan Xuquuqaha Way Dhawrsan Yihiin. | Khariidadda goobta Raadinta ugu Sareysa Telefoon |
| Tel | 86-18880477902 | Javanese | Iimayl |
| 8618880477902 | Shiinees | Faransiis | |
| Bird | Shiinees | Faransiis | Jarmal Isbaanish |
| Aquatic animals | Jabbaan | Kuuriyaan | Carabi Giriig |
| Turkiga | Talyaani | ||
| Ruminant animal g/head day | January 0.75 | Indoneesiya Afrikaans Iswiidhish |
Boolish ah
- Basque
- Catalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgaariya
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatian
Nederlandays
| Application object | Urdu Fiitnaam | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haytiyaan | Hausa | Kinyarwanda Hmong Hungarian |
| Piglets and fattening pigs | Igbo | Javanese | Kanada Khmer Kurdish |
| Kirgiz | Laatiin | ||
| Bird | 300～400 | 45～60 | Macedoniya Malaay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Noorwiijiyan
- Bashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbian
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Sawaaxili
Tajik
Tamil
Telugu
Tayland
| Application object | Urdu Fiitnaam | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yurub | Zulu | Kinyarwanda Oriya Turkmenistan |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
