Hordhac yar oo Peptide Raac Chelates Macdanta
Qaybta 1aad Taariikhda Raad-raaca Macdanta lagu daro
Waxaa loo qaybin karaa afar jiil iyadoo loo eegayo horumarinta waxyaabaha lagu daro macdanta:
Jiilka koowaad: Cusbada aan organic ahayn ee macdanta raadraaca, sida copper sulfate, ferrous sulfate, zinc oxide, iwm; Jiilka labaad: cusbada aashitada organic ee macdanta raadraaca, sida lactate ferrous, fumarate ferrous, citrate copper, iwm.; Jiilka saddexaad: Amino acid chelate waxay quudiyaan heerka macdanta raadraaca, sida zinc methionine, glycine iron iyo zinc glycine; Jiilka afraad: cusbada borotiinka iyo peptide yar oo chelating milix ah oo macdanta raad raac ah, sida copper protein, iron protein, zinc protein, manganese protein, copper peptide yar, iron peptide yar, zinc peptide yar, manganese peptide yar, iwm.
Jiilka koowaad waa macdanta raadraaca aan organic, jiilka labaad ilaa afraadna waa macdanta raadraaca dabiiciga ah.
Qaybta 2 Waa maxay sababta loo doorto Chelates Peptide Yaryar
Chelates peptide yar ayaa leh waxtarka soo socda:
1. Marka peptides-yar-yar ay chelate ions bir ah, waxay qani ku yihiin foomamka oo ay adagtahay in la buuxiyo;
2. Kuma tartanto kanaalada amino acid, waxay leedahay meelo badan oo nuugista iyo xawaaraha nuugista degdega ah;
3. Isticmaalka tamarta yar; 4. Dhignaasho badan, heerka isticmaalka sare iyo wax soo saarka xoolaha oo aad u wanaajiyay;
5. Ka-hortagga bakteeriyada iyo antioxidant;
6. Nidaaminta difaaca jirka.
Tiro badan oo daraasado ah ayaa muujiyay in sifooyinka kor ku xusan ama saamaynta chelates peptide yar ay ka dhigayaan inay leeyihiin rajooyin codsi oo ballaadhan iyo suurtagalnimada horumarinta, sidaas darteed shirkadeena ayaa ugu dambeyntii go'aansatay in ay qaadato chelates peptide yar sida diiradda cilmi-baarista iyo horumarinta alaabta macdanta dabiiciga ah ee shirkadda.
Qaybta 3 Waxtarka chelates peptide yar yar
1.Xiriirka ka dhexeeya peptides, amino acids iyo borotiinada
Miisaanka molecular ee borotiinku wuxuu ka badan yahay 10000;
Miisaanka molecular ee peptide waa 150 ~ 10000;
peptides yaryar, oo sidoo kale loo yaqaan peptides molecular yar, waxay ka kooban yihiin 2 ~ 4 amino acids;
Celcelis ahaan miisaanka unugyadu ee amino acids waa ilaa 150.
2. Isku-dubbaridka kooxaha amino acids iyo peptides oo biraha lagu jeexjeexay
(1) Isku-dubbaridka kooxaha ku jira amino acids
Isku xirka kooxaha ku jira asiidhyada amino:
Amino iyo kooxaha karboksyl oo saaran a-carbon;
Kooxaha silsiladaha ee qaar ka mid ah a-amino acids, sida kooxda sulfhydryl ee cysteine, kooxda phenolic ee tyrosine iyo kooxda imidazole ee histidine.
(2) Isku-dubbaridka kooxaha ee peptides yaryar
peptides-yar-yar waxay leeyihiin kooxo isku-dubbarid badan marka loo eego amino acids. Marka ay chelate ions bir ah, way fududahay in la chelate, oo waxay samayn karaan chelate multidentate, taas oo ka dhigaysa chelate mid deggan.
3. Waxtarka sheyga yar yar ee peptide chelate
Saldhig aragtiyeed ee peptide yar oo kor u qaada nuugista macdanta raadraaca
Astaamaha nuugista ee peptides yar yar ayaa ah aasaaska aragtida ee kor u qaadista nuugista walxaha raadraaca. Marka loo eego aragtida dheef-shiid kiimikaadka borotiinka, waxa xayawaanku u baahan yihiin borotiinka waa waxa ay uga baahan yihiin asiidhyada amino ee kala duwan. Si kastaba ha ahaatee, sanadihii ugu dambeeyay, cilmi-baaristu waxay muujisay in saamiga isticmaalka amino acids ee quudinta laga helo ilo kala duwan ay kala duwan tahay, iyo marka xayawaanka lagu quudiyo cunto homozygous ah ama cunto dheellitiran oo amino acid ah oo hooseeya, waxqabadka wax soo saarka ugu fiican lama heli karo (Baker, 1977; Pinchasov et al., 1990) [2,3]. Sidaa darteed, culimada qaarkood waxay soo bandhigeen aragtida ah in xayawaanku ay leeyihiin awood nuugista gaarka ah ee borotiinka laftiisa ama peptides la xidhiidha. Agar (1953) [4] ayaa markii ugu horreysay arkay in mareenka mindhicirku uu si buuxda u nuugi karo oo uu qaadi karo diglycidyl. Tan iyo markaas, cilmi-baarayaashu waxay soo bandhigeen dood lagu qanci karo oo ah in peptides yar yar si buuxda loo nuugo, iyaga oo xaqiijinaya in glycylglycine aan qummanayn la qaado oo la nuugo; Tiro badan oo peptides yar yar ayaa si toos ah loogu nuugi karaa wareegga nidaamka ee qaabka peptides. Hara iyo al. (1984) [5] ayaa sidoo kale tilmaamay in borotiinka dheef-shiid kiimikaadkiisa ku jira habka dheef-shiidka ay u badan yihiin peptides yaryar halkii ay ka ahaan lahaayeen amino acids (FAA). peptides-yada yaryar waxay si buuxda u dhex mari karaan unugyada xuubka mindhicirka waxayna galaan wareegga nidaamka (Le Guowei, 1996) [6].
Horumarka Cilmi-baarista ee Peptide-yar ee Kor-u-qaadista Nuugista Macdanta Raadka, Qiao Wei, et al.
Chelates peptide yar yar ayaa la qaadaa oo lagu nuugaa qaab peptides yar yar
Sida laga soo xigtay habka nuugista iyo gaadiidka iyo sifooyinka peptides yar yar, macdanta raadku waxay chelate leh peptides yar yar sida ligands ugu weyn ayaa laga yaabaa in la qaado guud ahaan, taas oo aad u fiican si loo hagaajiyo awoodda noolaha ee macdanta raadraaca. (Qiao Wei, iyo al)
Waxtarka Chelates Peptide Yaryar
1. Marka peptides-yar-yar ay chelate ions bir ah, waxay qani ku yihiin foomamka oo ay adagtahay in la buuxiyo;
2. Kuma tartanto kanaalada amino acid, waxay leedahay meelo badan oo nuugista iyo xawaaraha nuugista degdega ah;
3. Isticmaalka tamarta yar;
4. Dhignaasho badan, heerka isticmaalka sare iyo wax soo saarka xoolaha oo aad u wanaajiyay;
5. Ka-hortagga bakteeriyada iyo antioxidant; 6. Nidaaminta difaaca jirka.
4. Fahamka dheeraadka ah ee peptides
Keebaa labada isticmaale ee peptide-ka u hela lacag badan?
- peptide xidha
- Phosphopeptide
- reagents la xidhiidha
- Antimicrobial peptide
- difaaca jirka peptide
- Neuropeptide
- Hormoonka peptide
- Antioxidant peptide
- Peptides nafaqeynta
- Maaddada peptides
(1) Kala soocidda peptides
(2) Saamaynta jireed ee peptides
- 1. Hagaajin dheelitirka biyaha iyo korantada ee jirka;
- 2. Samee unugyada difaaca jirka ee ka hortaga bakteeriyada iyo caabuqa habka difaaca si uu u hagaajiyo shaqada difaaca;
- 3. Kor u qaadida bogsashada dhaawaca; Dayactirka degdega ah ee dhaawaca unugyada epithelial.
- 4. Samaynta enzymes ee jidhka waxay caawisaa inay cuntada u beddesho tamar;
- 5. Hagaajinta unugyada, hagaajinta dheef-shiid kiimikaadka unugyada, ka hortagga burburka unugyada, waxayna door ka ciyaaraan ka hortagga kansarka;
- 6. Kor u-qaadista iyo habaynta borotiinka iyo enzymes;
- 7. Ergey kiimiko ah oo muhiim ah si uu u gudbiyo macluumaadka u dhexeeya unugyada iyo xubnaha;
- 8. Ka hortagga cudurrada wadnaha iyo xididdada dhiigga;
- 9. Habeeya habdhiska qanjidhada qanjirada iyo habdhiska dareenka.
- 10. Hagaajinta hab-dhiska dheefshiidka iyo daawaynta cudurrada daba-dheeraada ee caloosha;
- 11. Hagaajinta macaanka, laabotooyinka, laabotooyinka iyo cudurrada kale.
- 12. Infekshanka ka hortagga fayraska, ka-hortagga gabowga, baabi'inta xag-jirnimada xad-dhaafka ah ee jirka.
- 13. Kor u qaadida shaqada hematopoietic, daawaynta dhiig-yarida, ka hortagga isku-ururka platelet, kaas oo wanaajin kara awoodda qaadista ogsajiinta ee unugyada dhiigga cas ee dhiigga.
- 14. Si toos ah ula dagaallan fayraska DNA-ga oo bar-tilmaameedsada bakteeriyada fayraska.
5. Shaqada nafaqeynta laba-geesoodka ah ee chelates peptide yar
Chelate peptide yar wuxuu galaa unuga guud ahaan jirka xayawaanka, iyoka dibna si toos ah u jebiya curaarta chelation-kaunugga oo u kala go'a peptide iyo ion bir ah, kuwaas oo siday u kala horreeyaan loo isticmaaloxayawaanka si uu u ciyaaro laba hawlood oo nafaqeyn ah, gaar ahaandoorka functional peptide.
Shaqada peptide yar yar
- 1. Kor u qaadista borotiinka ee unugyada muruqa xayawaanka, yaraynta apoptosis, iyo kor u qaadista koritaanka xayawaanka
- 2.Hagaajinta qaabka flora mindhicirka iyo kor u qaadida caafimaadka mindhicirka
- 3. Bixi qalfoofka kaarboonka oo kordhiya dhaqdhaqaaqa enzymes dheefshiidka sida amylase xiidmaha iyo protease
- 4.Waxay leedahay saameyn liddi ku ah diiqada
- 5. Leh sifooyinka anti-bararka
- 6……
6. Faa'iidooyinka chelates peptide yar yar ee chelates amino acids
| Amino acid chelated macdanta raadraaca | Maaddooyinka raadraaca ee peptide yar yar | |
| Qiimaha alaabta ceeriin | Alaabada ceeriin ee amino acids waa qaali | Alaabta ceeriin ee keratin ee Shiinaha ayaa aad u badan. Timaha, qoobabka iyo geesaha xanaanada xoolaha iyo borotiinka biyaha wasakhda ah iyo haragga hargaha ee warshadaha kiimikaad waa alaab cayriin borotiin ah oo tayo sare leh. |
| Saamaynta nuugista | Kooxaha Amino iyo Carboxyl waxay isku mar ku lug leeyihiin chelation of amino acids iyo walxaha birta, samaynta qaab-dhismeedka endocannabinoid bicyclic oo la mid ah kan dipeptides, oo aan lahayn kooxo karboksyl ah oo bilaash ah, kaas oo kaliya lagu nuugi karo nidaamka oligopeptide. (Su Chunyang et al., 2002) | Marka peptides yar yar ay ka qayb qaataan chelation, hal giraan qaab dhismeedka chelation guud ahaan waxaa sameeyay kooxda amino terminal iyo peptide bond ogsijiinta ku dhow, iyo chelate wuxuu hayaa koox kaarboxyl ah oo lacag la'aan ah, kaas oo lagu nuugi karo iyada oo loo marayo nidaamka dipeptide, oo leh nuugista aad uga sarreeya nidaamka oligopeptide. |
| Xasilooni | Iions bir ah oo leh hal ama in ka badan oo shan-xubnood ah ama lix-xubnood ah oo siddo oo ka kooban kooxaha amino, kooxaha karboxyl, kooxaha imidazole, kooxaha phenol, iyo kooxaha sulfhydryl. | Marka lagu daro shanta kooxood ee isuduwidda ee amino acids, carbonyl iyo kooxaha imino ee peptides yaryar ayaa sidoo kale ku lug yeelan kara isuduwidda, sidaas darteed samaynta peptide chelates yaryar oo ka xasilan chelates amino acids.(Yang Pin et al., 2002) |
7. Faa'iidooyinka chelates peptide yar yar ee glycolic acid iyo methionine chelates
| Macdanta raadraaca ee Glycine cheated | Methionine chelated macdanta raadraaca | Maaddooyinka raadraaca ee peptide yar yar | |
| Foomka isuduwidda | Carboxyl iyo kooxaha amino ee glycine waxaa la isku dubaridi karaa ion bir ah. | Carboxyl iyo kooxaha amino ee methionine waxa lagu xidhi karaa ion bir ah. | Marka la jeexjeexo ion bir ah, waxay qani ku tahay foomamka isuduwidda oo aan si fudud loo buuxin. |
| Shaqada nafaqada | Noocyada iyo hawlaha amino acids waa hal. | Noocyada iyo hawlaha amino acids waa hal. | Thekala duwan oo qani ahAsiidhyada amino-yada waxay bixiyaan nafaqo aad u dhammaystiran, halka peptides-yada yaryari ay si habboon u shaqayn karaan. |
| Saamaynta nuugista | Chelates Glycine waxay leeyihiinnoKooxaha karboksylka ee bilaashka ah ayaa jooga waxayna leeyihiin saamayn nuugista oo gaabis ah. | Methionine chelates ayaa lehnoKooxaha karboksylka ee bilaashka ah ayaa jooga waxayna leeyihiin saamayn nuugista oo gaabis ah. | Chelates peptide yar ayaa sameeyayka koobanjoogitaanka kooxaha karboxyl ee bilaashka ah waxayna leeyihiin saameyn nuugista degdega ah. |
Qaybta 4 Magaca Ganacsiga "Peptide-mineral Chelates Yaryar"
Chelates Peptide-mineral-ka yar, sida magacaba ka muuqata, way fududahay in la khiyaamiyo.
Waxay ka dhigan tahay ligands peptide yar yar, kuwaas oo aan si fudud loo buuxin sababtoo ah tirada badan ee kooxaha iskudubarid, Si fudud loo sameeyo chelate multidentate oo leh walxo bir ah, oo leh xasilooni wanaagsan.
Qaybta 5 Hordhaca Alaabooyinka Taxanaha ah ee Peptide-mineral Chelates
1. Macdanta peptide yar oo naxaas ah (magaca ganacsiga: Copper Amino Acid Chelate Feed Grade)
2. Raad peptide yar oo macdanta birta ah (magaca ganacsiga: Ferrous Amino Acid Chelate Feed Grade)
3. Macdanta peptide yar oo chelated zinc (magaca ganacsiga: Zinc Amino Acid Chelate Feed Grade)
4. Raad peptide yar oo macdanta manganese ah (magaca ganacsiga: Manganese Amino Acid Chelate Feed Grade)
Naxaasta Amino Acid Chelate Feed Fasalka
Ferrous Amino Acid Chelate Feed Fasalka
Zinc Amino Acid Chelate Feed Fasalka
Manganese Amino Acid Chelate Feed Fasalka
1. Fasalka Quudinta Amino Acid Chelate Feed
- Magaca Alaabta: Fasalka Quudinta Amino Acid Chelate Copper
- Muuqashada: granules cagaaran brownish
- Halbeegyada physicochemical
a) Copper: ≥ 10.0%
b) Wadarta amino acids: ≥ 20.0%
c) Heerka Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Ledhka: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Waxa ku jira qoyaanka: ≤ 5.0%
h) Wanaajinta: Dhammaan qaybaha waxay maraan 20 mesh, oo leh cabbirka walxaha ugu muhiimsan ee 60-80 mesh
n=0,1,2,...waxay tusinaysaa naxaasta chelated ee dipeptides, tripeptides, iyo tetrapeptides
Diglycerin
Qaab dhismeedka chelates peptide yar yar
Astaamaha darajada Quudinta ee Amino Acid Chelate Feed
- Alaabtani waa macdan raad-raac oo dhan-organic ah oo ay farsameeyaan habab chelating gaar ah oo leh geed saafi ah enzymatic molecule peptides yaryar
- Alaabtani waa mid kiimiko ahaan degan waxayna si weyn u yarayn kartaa waxyeeladeeda fiitamiinada iyo dufanka, iwm.
- Isticmaalka alaabtani waxay ku haboon tahay hagaajinta tayada quudinta. Alaabta waxaa lagu nuugaa iyada oo loo marayo waddooyinka peptide yar iyo amino acid, yaraynta tartanka iyo ka soo horjeeda walxaha kale ee raadraaca, waxayna leedahay nuugista bio-nuugista iyo isticmaalka ugu fiican.
- Copper waa qaybta ugu muhiimsan ee unugyada dhiigga cas, unugyada isku xiran, lafaha, oo ku lug leh jirka noocyo kala duwan oo enzymes ah, kor u qaadida shaqada difaaca jirka, saameynta antibiyootiga, waxay kordhin kartaa miisaanka maalinlaha ah, hagaajinta gunnada quudinta.
Isticmaalka iyo waxtarka darajada Quudinta Chelate Amino Acid
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t walxo qiimo buuxa leh) | Waxa ku jira quudinta qiima buuxda (mg/kg) | Waxtarka |
| Sow | 400-700 | 60-105 | 1. Horumarinta waxqabadka taranka iyo ka faa'iidaysiga sannadaha abuurka; 2. Kordhi firfircoonida uurjiifka iyo doofaarka; 3. Hagaajinta difaaca iyo caabbinta cudurada. |
| Doofaar | 300-600 | 45-90 | 1. Faa'iido u leh hagaajinta hawlaha hematopoietic iyo difaaca, kor u qaadida iska caabinta cadaadiska iyo caabbinta cudurada; 2. Kordhi heerka korriinka oo si weyn u wanaajiso waxtarka quudinta. |
| Doofaarka naaxinta | 125 | Janaayo 18.5 | |
| Shimbir | 125 | Janaayo 18.5 | 1. Hagaajinta iska caabbinta walbahaarka iyo dhimista dhimashada; 2. Hagaajinta magdhowga quudinta iyo kordhinta heerka koritaanka. |
| Xoolaha biyaha | Kalluunka 40～70 | 6 ilaa 10.5 | 1. Kor u qaadida koritaanka, hagaajinta magdhowga quudinta; 2. Ka-hortagga walbahaarka, waxay yaraysaa cudurrada iyo dhimashada. |
| Shilinka 150-200 | 22.5 ilaa 30 | ||
| Maalinta madaxa xoolaha ee caanka ah | Janaayo 0.75 | 1. Ka hortag qallafsanaanta wadajirka ah ee tibial, khalkhalka dhaqdhaqaaqa "dib-u-xiran", wobbler, dhaawac muruqa wadnaha; 2. Ka hortag timaha ama keratinization jaakad, noqo timo adag, lumin curvature caadiga ah, ka hortagga soo bixitaanka "dhibcaha cawl" ee goobada isha; 3. Ka hortag miisaanka dhimista, shubanka, hoos u dhaca wax soo saarka caanaha. |
2. Fasalka Quudinta Ferrous Amino Acid Chelate Feed
- Magaca Alaabta: Feerous Amino Acid Chelate Feed Fasalka
- Muuqashada: granules cagaaran brownish
- Halbeegyada physicochemical
a) Birta: ≥ 10.0%
b) Wadarta amino acids: ≥ 19.0%
c) Heerka Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Ledhka: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Waxa ku jira qoyaanka: ≤ 5.0%
h) Wanaajinta: Dhammaan qaybaha waxay maraan 20 mesh, oo leh cabbirka walxaha ugu muhiimsan ee 60-80 mesh
n=0,1,2,...waxay tusinaysaa zinc chelated ee dipeptides, tripeptides, iyo tetrapeptides
Sifooyinka Feerrous Amino Acid Chelate Feed Fasalka
- Alaabtani waa macdan raad-raac dabiici ah oo ay habeeyaan habraac gaar ah oo chelating leh oo leh warshad saafi ah enzymatic peptides molecule yar oo ah substrates chelating iyo walxaha raadraaca;
- Alaabtani waa mid kiimiko ahaan deggan waxayna si weyn u yarayn kartaa waxyeeladeeda fiitamiinada iyo dufanka, iwm.
- Badeecada waxaa lagu nuugaa iyada oo loo marayo dariiqyada peptide yar yar iyo amino acids, yaraynta tartanka iyo ka soo horjeeda walxaha kale ee raadraaca, waxayna leedahay nuugista bio-nuugista iyo isticmaalka ugu fiican;
- Alaabtani waxay ka gudbi kartaa xannibaadda mandheerta iyo qanjirka naasaha, waxay ka dhigi kartaa uurjiifka mid caafimaad qaba, kordhinta miisaanka dhalashada iyo miisaanka naaska, waxayna yareysaa heerka dhimashada; Birtu waa qayb muhiim ah oo ka mid ah haemoglobin iyo myoglobin, taas oo si wax ku ool ah uga hortagi karta dhiig-yaraanta birta iyo dhibaatooyinka ay keento.
Isticmaalka iyo waxtarka Fasalka Quudinta Ferrous Amino Acid Chelate Feed
| Shayga codsiga | Qiyaasta lagu taliyey (g/t alaab qiimo buuxda leh) | Waxa ku jira quudinta qiima buuxda (mg/kg) | Waxtarka |
| Sow | 300-800 | 45 ilaa 120 | 1. Horumarinta waxqabadka taranka iyo nolosha ka faa'iidaysiga abuurka; 2. wanaajisa miisaanka dhalashada, naaska naaska iyo isku midnimada doofaarka si loo helo waxqabad wax soo saar oo wanaagsan xilliga dambe; 3. Hagaajinta kaydinta xadiidka ee doofaarka nuujinaysa iyo xooga saarista birta ee caanaha si looga hortago dhiig yarida birta ee jaqa doofaarka. |
| Doofaarka iyo naaxinta doofaarka | Piglets 300-600 | 45-90 | 1. Hagaajinta difaaca doofaarka, kor u qaadista caabbinta cudurka iyo hagaajinta heerka badbaadada; 2. Kordhi heerka korriinka, hagaajinta beddelka quudinta, kordhiso miisaanka qashinka naaska iyo isku-duubnida, iyo yaraynta dhacdooyinka doofaarka; 3. Hagaajinta heerka myoglobin iyo myoglobin, ka hortagga iyo daawaynta dhiig-yaraanta birta, ka dhig maqaarka doofaarka mid guduudan oo si cad u wanaaji midabka hilibka. |
| Doofaarka naaxinta 200-400 | 30-60 | ||
| Shimbir | 300-400 | 45-60 | 1. Hagaajinta beddelka quudinta, kordhiso heerka korriinka, wanaaji awoodda ka-hortagga walbahaarka iyo dhimista dhimashada; 2. Hagaajinta heerka ugxanta dhalidda, yaree heerka jaban ee ukunta oo qoto midabka jaallada; 3. Hagaajinta heerka bacriminta iyo heerka dillaaca ukunta taranta iyo heerka badbaadada digaaga da'da yar. |
| Xoolaha biyaha | 200-300 | 30-45 | 1. Kor u qaadida koritaanka, hagaajinta beddelka quudinta; 2. Hagaajinta ka-hortagga walbahaarka, yaraynta cudurrada iyo dhimashada. |
3. Fasalka Quudinta Zinc Amino Acid Chelate Feed
- Magaca Alaabta: Fasalka Quudinta Amino Acid Chelate
- Muuqaal: granules brownish-jaalle
- Halbeegyada physicochemical
a) Zinc: ≥ 10.0%
b) Wadarta amino acids: ≥ 20.5%
c) Heerka Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Ledhka: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Waxa ku jira qoyaanka: ≤ 5.0%
h) Wanaajinta: Dhammaan qaybaha waxay maraan 20 mesh, oo leh cabbirka walxaha ugu muhiimsan ee 60-80 mesh
n=0,1,2,...waxay tusinaysaa zinc chelated ee dipeptides, tripeptides, iyo tetrapeptides
Sifooyinka Zinc Amino Acid Chelate Feed Fasalka
Alaabtani waa macdan raad-raac oo dhan-organic ah oo lagu farsameeyay hab-raac gaar ah oo leh geedka saafiga ah ee enzymatic peptides molecule yaryar sida substrates chelating iyo walxaha raadraaca;
Alaabtani waa mid kiimiko ahaan degan waxayna si weyn u yarayn kartaa waxyeeladeeda fiitamiinada iyo dufanka, iwm.
Isticmaalka alaabtani waxay ku haboon tahay hagaajinta tayada quudinta; Badeecada waxaa lagu nuugaa iyada oo loo marayo dariiqyada peptide yar yar iyo amino acids, yaraynta tartanka iyo ka soo horjeeda walxaha kale ee raadraaca, waxayna leedahay nuugista bio-nuugista iyo isticmaalka ugu fiican;
Alaabtani waxay wanaajin kartaa difaaca, kor u qaadida koritaanka, kordhinta beddelka quudinta iyo hagaajinta dhalaalka dhogorta;
Zinc waa qayb muhiim ah oo ka kooban in ka badan 200 enzymes, unug epithelial, ribose iyo gustatin. Waxay kor u qaadaysaa kororka degdega ah ee unugyada burooyinka dhadhanka ee xuubka carrabka waxayna nidaamisaa rabitaanka cuntada; waxay joojisaa bakteeriyada waxyeelada leh ee mindhicirka; waxayna leedahay shaqada antibiyootiga, taas oo hagaajin karta shaqada dheecaanka ee habka dheef-shiidka iyo dhaqdhaqaaqa enzymes ee unugyada iyo unugyada.
Isticmaalka iyo waxtarka Zinc Amino Acid Chelate Feed Fasalka
| Shayga codsiga | Qiyaasta lagu taliyey (g/t alaab qiimo buuxda leh) | Waxa ku jira quudinta qiima buuxda (mg/kg) | Waxtarka |
| Beeridda uurka leh iyo kuwa nuujinaya | 300-500 | 45-75 | 1. Horumarinta waxqabadka taranka iyo nolosha ka faa'iidaysiga abuurka; 2. Hagaajinta tamarta uurjiifka iyo doofaarka, kor u qaad iska caabbinta cudurrada, oo ka dhig inay yeeshaan waxqabad wax soo saar oo wanaagsan marxaladda dambe; 3. Hagaajinta xaalada jireed ee abuurka uurka leh iyo miisaanka dhalashada ee doofaarka. |
| Nuugista doofaarka, doofaarka iyo doofaarrada koraya-naaxinaya | 250-400 | 37.5 ilaa 60 | 1. Horumarinta difaaca doofaarka, yaraynta shubanka iyo dhimashada; 2. Hagaajinta dhadhanka, kordhinta qaadashada quudinta, kordhinta heerka koritaanka iyo hagaajinta beddelka quudinta; 3. Ka dhig jaakada doofaarka mid dhalaalaya oo wanaaji tayada hilibka iyo tayada hilibka. |
| Shimbir | 300-400 | 45-60 | 1. Hagaajinta dhalaalka baalasha; 2. hagaajinta heerka dhalmada, heerka bacriminta iyo heerka dillaaca ukunta, iyo xoojinta awoodda midabaynta ukunta ukunta; 3. Hagaajinta awoodda ka-hortagga walbahaarka iyo dhimista dhimashada; 4. Hagaajinta beddelka quudinta oo kordhi heerka koritaanka. |
| Xoolaha biyaha | Janaayo 300 | 45 | 1. Kor u qaadida koritaanka, hagaajinta beddelka quudinta; 2. Hagaajinta ka-hortagga walbahaarka, yaraynta cudurrada iyo dhimashada. |
| Maalinta madaxa xoolaha ee caanka ah | 2.4 | 1. Hagaaji dhalidda caanaha, ka hortag naasaha iyo qudhunka foofka, oo ay yareeyaan waxa ku jira unugyada somatic ee caanaha; 2. Kor u qaadida koritaanka, wanaaji beddelka quudinta iyo hagaajinta tayada hilibka. |
4. Manganese Amino Acid Chelate Feed Fasalka
- Magaca Alaabta: Manganese Amino Acid Chelate Feed Fasalka
- Muuqaal: granules brownish-jaalle
- Halbeegyada physicochemical
a) Mn: ≥ 10.0%
b) Wadarta amino acids: ≥ 19.5%
c) Heerka Chelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Ledhka: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Waxa ku jira qoyaanka: ≤ 5.0%
h) Wanaajinta: Dhammaan qaybaha waxay maraan 20 mesh, oo leh cabbirka walxaha ugu muhiimsan ee 60-80 mesh
n=0, 1,2,...waxay tusinaysaa manganese chelated ee dipeptides, tripeptides, iyo tetrapeptides
Sifooyinka Manganese Amino Acid Chelate Feed Fasalka
Alaabtani waa macdan raad-raac oo dhan-organic ah oo lagu farsameeyay hab-raac gaar ah oo leh geedka saafiga ah ee enzymatic peptides molecule yaryar sida substrates chelating iyo walxaha raadraaca;
Alaabtani waa mid kiimiko ahaan deggan waxayna si weyn u yarayn kartaa waxyeeladeeda fiitamiinada iyo dufanka, iwm.
Badeecada waxaa lagu nuugaa iyada oo loo marayo dariiqyada peptide yar yar iyo amino acids, yaraynta tartanka iyo ka soo horjeeda walxaha kale ee raadraaca, waxayna leedahay nuugista bio-nuugista iyo isticmaalka ugu fiican;
Alaabtu waxay wanaajin kartaa heerka korriinka, waxay hagaajin kartaa beddelka quudinta iyo heerka caafimaadka si weyn; iyo kor u qaadida heerka dhalmada, heerka dillaacinta iyo heerka digaagga ee caafimaadka leh ee taranta sida cad;
Manganese waxay lagama maarmaan u tahay koritaanka lafaha iyo ilaalinta unugyada isku xiran. Waxay si dhow ula xiriirtaa enzymes badan; oo ka qaybqaata karbohaydrayt, dufanka iyo dheef-shiid kiimikaadka borotiinka, taranka iyo jawaab celinta difaaca.
Isticmaalka iyo waxtarka Manganese Amino Acid Chelate Feed Fasalka
| Shayga codsiga | Qiyaasta la soo jeediyay (g/t walxo qiimo buuxa leh) | Waxa ku jira quudinta qiima buuxda (mg/kg) | Waxtarka |
| Doofaarka taranta | 200-300 | 30-45 | 1. Kor u qaadida horumarka caadiga ah ee xubnaha galmada iyo hagaajinta dhaqdhaqaaqa shahwada; 2. Hagaajinta awoodda taranka ee taranta doofaarka iyo in la yareeyo caqabadaha taranka. |
| Doofaarka iyo naaxinta doofaarka | 100-250 | 15-37.5 | 1. Waxay faa'iido u leedahay in la wanaajiyo hawlaha difaaca, iyo wanaajinta awoodda ka hortagga cadaadiska iyo caabbinta cudurrada; 2. Kor u qaadida koritaanka iyo hagaajinta beddelka quudinta si weyn; 3. Hagaaji midabka iyo tayada hilibka, oo hagaaji boqolkiiba hilibka caatada ah. |
| Shimbir | 250-350 | 37.5-52.5 | 1. Hagaajinta awoodda ka-hortagga walbahaarka iyo dhimista dhimashada; 2. Hagaajinta heerka dhalidda, heerka bacriminta iyo heerka dillaaca ukunta taranka, wanaaji tayada qolofta ukunta oo yaraynaysa heerka jebinta qolofka; 3. Kor u qaadida korriinka lafaha iyo in la yareeyo dhacdooyinka cudurrada lugaha. |
| Xoolaha biyaha | 100-200 | 15 ilaa 30 | 1. Kor u qaadida korriinka oo wanaajiso awooddeeda ka hortagga walbahaarka iyo iska caabbinta cudurrada; 2. Hagaajinta dhaqdhaqaaqa shahwada iyo heerka dillaaca ukumaha la bacrimiyay. |
| Maalinta madaxa xoolaha ee caanka ah | Lo'da 1.25 | 1. Kahortagga khalkhal abuurka fatty acid iyo dhaawaca unugyada lafaha; 2. In kor loo qaado awoodda taranka, lagana hortago ilmo soo rididda iyo curyaanka dhalmada ka dib ee xoolaha dheddigga, yaraynta dhimashada weylaha iyo baraarka. iyo in la kordhiyo miisaanka dhasha ee xoolaha yaryar. | |
| Ari 0.25 |
Qaybta 6 FAB ee Chelates-yar ee Peptide-macdanta
| S/N | F: Sifooyin shaqaynaya | J: Kala duwanaanshaha tartanka | B: Faa'iidooyinka ay keeneen kala duwanaanshiyaha tartanka ee isticmaalayaasha |
| 1 | Xakamaynta xulashada alaabta ceeriin | Dooro dhirta saafiga ah enzymatic hydrolysis ee peptides yar yar | Badbaadada nafleyda oo sarreeya, ka fogaanshaha dadka cunista |
| 2 | Farsamada dheefshiidka jihada ee borotiinka laba-laaban ee enzyme noole | Qayb sare oo ah peptides molecular yar yar | "Bartilmaameedyo" badan, kuwaas oo aan si fudud loo buuxin, oo leh dhaqdhaqaaq bayooloji oo sarreeya iyo xasillooni wanaagsan |
| 3 | buufinta cadaadiska horumarsan & farsamada qalajinta | Alaabta granular, oo leh cabbirka walxaha isku midka ah, dareere wanaagsan, ma fududa in la nuugo qoyaanka | Hubi in ay fududahay in la isticmaalo, isku darka labbiska badan ee quudinta dhamaystiran |
| Biyaha ku jira oo hooseeya (≤ 5%), taas oo si weyn u yaraynaysa saamaynta ay keenaan fiitamiinnada iyo diyaarinta enzymes | Hagaajinta xasiloonida alaabta quudinta | ||
| 4 | Farsamada xakamaynta wax soo saarka sare | Habka gebi ahaanba xiran, heer sare oo xakameyn toos ah | Tayo sugan oo deggan |
| 5 | Farsamada xakamaynta tayada horumarsan | Samee oo wanaaji hababka falanqaynta sayniska iyo sare ee sayniska iyo habka xakamaynta ee lagu ogaanayo arrimaha saameeya tayada alaabta, sida borotiinka-milmi kara, qaybinta miisaanka unugyada, amino acids iyo heerka chelating | Hubi tayada, hubi hufnaanta oo wanaaji hufnaanta |
Qaybta 7 Isbarbardhigga Tartamayaasha
Heerka VS Heerka
Isbarbardhigga qaybinta peptide iyo heerka chelation ee alaabta
| Alaabooyinka Sustar | Qaybta peptides yar yar (180-500) | Alaabada Zinpro | Qaybta peptides yar yar (180-500) |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Alaabooyinka Sustar | Heerka Chelation | Alaabada Zinpro | Heerka Chelation |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
Saamiga peptides-ka yar yar ee Sustar wax yar ayuu ka hooseeyaa kan Zinpro, iyo heerka chelation-ka ee badeecadaha Sustar wax yar ayuu ka sarreeyaa kan alaabta Zinpro.
Isbarbardhigga waxa ku jira 17 amino acids oo ku jira alaabooyin kala duwan
| Magaca amino acids | Sustar's Copper Amino Acid Chelate Fasalka Quudinta | Zinpro's AVAILA naxaas | Sustar's Ferrous Amino Acid C helate Feed Darajo | Zinpro's AVAILA birta | Manganese Sustar Amino Acid Chelate Fasalka Quudinta | Zinpro's AVAILA manganese | Sustar's Zinc Amino acid Chelate Feed Fasalka | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tirosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Janaayo 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Wadarta amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Guud ahaan, saamiga amino acids ee alaabta Sustar ayaa ka sarreeya kuwa Zinpro's.
Qaybta 8 Saamaynta isticmaalka
Saamaynta ilaha kala duwan ee macdanta raadku ku leeyihiin waxqabadka wax soo saarka iyo tayada ugxanta digaaga ee xilliga jiifka dambe
Habka wax soo saarka
- Tignoolajiyada chelation ee la beegsaday
- Tignoolajiyada emulsification xiirid
- Buufin cadaadiska & farsamada qalajinta
- Tignoolajiyada qaboojiyaha & qoyaan-dejinta
- Farsamada ilaalinta deegaanka ee horumarsan
Lifaaqa A: Hababka lagu Go'aamiyo qaybinta cufka molecular qaraabo ee peptides
Qaadashada heerka: GB/T 22492-2008
1 Mabda'a Tijaabada:
Waxaa lagu go'aamiyay chromatography sifaynta gel waxqabadka sare. Taasi waa in la yidhaahdo, iyadoo la isticmaalayo buuxinta daloolka sida weji taagan, oo ku salaysan farqiga u size molecular qaraabo ah tiro cuf ee qaybaha muunad ee kala soocidda, lagu ogaaday bond peptide ee mawjada nuugista ultraviolet ee 220nm, iyadoo la isticmaalayo software xogta u go'an go'aaminta qaybinta mass molecular qaraabo by shaandhaynta gel chromatografi-ka software ah (ie, chromatography) xogta ay ahaayeen. la xisaabiyay si loo helo cabbirka cufka molecular qaraabo ee peptide soybean iyo kala qaybinta qaybinta.
2. Reagents
Biyaha tijaabada ahi waa inay buuxiyaan qeexida biyaha labaad ee GB/T6682, isticmaalka reagents, marka laga reebo qodobbo gaar ah, waa falanqayn ahaan saafi ah.
2.1 Reagents waxaa ka mid ah acetonitrile (chromatographically saafi ah), trifluoroacetic acid (chromatographically saafi ah),
2.2 Walxaha caadiga ah ee loo isticmaalo qalooca cabbirka ee qaybinta cufnaanta molecular qaraabo: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Qalab iyo qalab
3.1 Waxqabadka Sare ee Chromatograph (HPLC): goob shaqo chromatographic ama isku xidhe leh aaladaha UV iyo software habaynta xogta GPC.
3.2 unugga nadiifinta faakuumka wajiga wareega iyo nadiifinta.
3.3 dheelitirka elegtarooniga ah: qiimaha qalin jabiyay 0.000 1g.
4 Tallaabooyinka hawlgalka
4.1 Xaaladaha Chromatographic iyo tijaabooyinka la qabsiga nidaamka (xaaladaha tixraaca)
4.1.1 Tiirka Chromatographic: TSKgelG2000swxl300 mm × 7.8 mm (dhexroorka gudaha) ama tiirar kale oo jel oo isku mid ah oo leh waxqabad la mid ah oo ku habboon go'aaminta borotiinnada iyo peptides.
4.1.2 Wajiga guurguura: Acetonitrile + biyaha + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Dhererka mowjadda ogaanshaha: 220 nm.
4.1.4 Heerka socodka: 0.5 ml/min.
4.1.5 Waqtiga ogaanshaha: 30 min.
4.1.6 Tusaalaha mugga duridda: 20μL.
4.1.7 Heerkulka tiirka: heerkulka qolka.
4.1.8 Si loo sameeyo nidaamka chromatographic buuxiyo shuruudaha ogaanshaha, waxaa lagu qeexay in xaaladaha koromografiga ee kor ku xusan, waxtarka tiirarka chromatographic gel gel, ie, tirada teoretisk ee taarikada (N), aan ka yarayn 10000 oo la xisaabiyay iyadoo lagu saleynayo heerka ugu sarreeya ee heerka tripeptide (Glye-Glycine).
4.2 Soo saarista qoob-ka-cayaarka cufnaanta molecular qaraabo
Xalalka caadiga ah ee peptide mass molecular ee kor ku xusan oo leh uruurin ballaaran oo ah 1 mg / mL ayaa lagu diyaariyey isbarbardhigga wajiga wareega, lagu qaso qayb gaar ah, ka dibna lagu sifeeyay xuubka wejiga dabiiciga ah ee cabbirka daloolka 0.2 μm ~ 0.5 μm waxaana lagu duraa muunada, ka dibna chromatogram-yada heerarka ayaa la helay. Qalloocyada qiyaasida molecular-ka ee qaraabo iyo isla'egyadooda ayaa lagu helay iyada oo la qorshaynayo logarithm cufka molecular ee ka soo horjeeda wakhtiga haynta ama dib u noqoshada toosan.
4.3 Tusaalaha daawaynta
Si sax ah u miisaamay 10mg muunad in a 10mL musqul musqul, ku dar wajiga yar mobile, ultrasonic ruxruxo 10min, si muunad si buuxda u milmay oo isku qasan, qasi wajiga mobile in miisaanka, ka dibna sifeeyo iyada oo xuubka wajiga organic la size dalool ah 0.2μm ~ 0.5μm, iyo shaandhaynta waxaa lagu falanqeeyay chromographic 1.
5. Xisaabinta qaybinta molecular qaraabo ah
Ka dib marka la falanqeeyo saamiga muunada ee lagu diyaariyay 4.3 ee hoos imanaya xaaladaha chromatographic ee 4.1, cufnaanta molecular-ka ee muunadda iyo kala qaybinta qaybinteeda waxaa lagu heli karaa iyada oo lagu beddelayo xogta chromatographic ee muunada ee qalooca cabbirka 4.2 oo leh software farsamaynta xogta GPC. Qaybinta cufnaanta molecular-ka ee peptides kala duwan waxaa lagu xisaabin karaa habka caadiga ah ee aagga ugu sarreeya, sida waafaqsan qaacidada: X=A/A total×100
Qaaciddada: X - Tirada guud ee peptide cufnaanta molecular qaraabo ee wadarta peptide ee muunadda,%;
A - Meesha ugu sarreysa ee peptide mass molecular qaraabo;
Wadarta A - wadarta meelaha ugu sarreeya ee peptide cufka molecular kasta, oo lagu xisaabiyo hal jajab tobanle.
6 Ku celcelinta
Farqiga buuxa ee u dhexeeya laba go'aanno madax-bannaan oo lagu helay xaaladaha dib-u-celinta waa in aanu dhaafin 15% celceliska xisaabta ee labada go'aan.
Lifaaqa B: Hababka lagu Go'aamiyo Asiidhyada Amino ee Xorta ah
Qaadashada heerka: Q/320205 KAVN05-2016
1.2 Reagents iyo qalab
Glacial acetic acid: falanqayn ahaan saafi ah
Perchloric acid: 0.0500 mol/L
Tilmaame: 0.1% tilmaame violet crystal (glacial acetic acid)
2. Go'aaminta amino acids-ka bilaashka ah
Tijaabooyinku waxa lagu qallajiyey 80°C 1 saac.
Muunada ku rid weel qallalan si aad si dabiici ah ugu qaboojiso heerkulka qolka ama aad u qaboojiso heerkul la isticmaali karo.
Miisaam ku dhawaad 0.1 g oo muunad ah (sax ah ilaa 0.001 g) geli 250 mL weel qallalan oo kooniska ah.
Si degdeg ah ugu gudub talaabada xigta si aad uga fogaato muunada in ay nuugo qoyaanka deegaanka
Ku dar 25 ml oo ah glacial acetic acid oo si fiican isku qas wax aan ka badnayn 5 daqiiqo.
Ku dar 2 dhibcood oo tilmaame violet crystal ah
Titrate leh 0.0500 mol / L (± 0.001) xal titration caadiga ah ee perchloric acid ilaa xalku ka bedelayo guduudan ilaa dhamaadka barta.
Qor mugga xalka caadiga ah ee la isticmaalo.
Samee tijaabada maran isku mar.
3. Xisaabinta iyo natiijooyinka
Maaddada amino acid ee bilaashka ah ee ku jirta reagent-ka waxaa loo muujiyey sida jajab tiro badan (%) waxaana loo xisaabiyaa si waafaqsan qaacidada: X = C × (V1-V0) × 0.1445/M × 100%, ee qaaciidada tne:
C - Isku-duubnaanta xalalka aashitada perchloric ee caadiga ah ee moles halkii litir (mol/L)
V1 - mugga loo isticmaalo titration of shaybaarka leh xal perchloric acid caadiga ah, millilitir (ml).
Vo - mugga loo isticmaalo titration maran oo leh xalka perchloric acid caadiga ah, millilitir (mL);
M - Cufka muunada, garaam (g).
0.1445: Celceliska tirada amino acids ee u dhiganta 1.00 mL ee xalalka perchloric acid caadiga ah [c (HClO4) = 1.000 mol / L].
Lifaaqa C: Hababka lagu Go'aamiyo heerka Chelation Sustar
Qaadashada halbeegyada: Q/70920556 71-2024
1. Mabda'a go'aaminta (Fe tusaale ahaan)
Iskudhisyada birta ee Amino acid waxay leeyihiin milmayn aad u hooseeya ee ethanol anhydrous iyo ion birta bilaashka ah waxay ku milmayaan ethanol anhydrous, farqiga u dhexeeya milanka labada ee ethanol anhydrous ayaa loo adeegsaday si loo go'aamiyo heerka chelation ee dhismayaasha birta amino acid.
2. Reagent & Solutions
ethanol anhydrous; inta soo hartay waxay la mid tahay faqradda 4.5.2 ee GB/T 27983-2011.
3. Tallaabooyinka falanqaynta
Samee laba tijaabo oo isbarbar socda. Miisaanka 0.1g ee muunada la qalajiyey 103 ± 2 ℃ 1 saac, sax ah 0.0001g, ku dar 100mL of ethanol anhydrous si ay u milaan, filter, hadhaaga filter lagu dhaqay 100mL of ethanol anhydrous ugu yaraan saddex jeer, ka dibna ku wareeji haraaga galay 250m acid flask ah. sida ku cad faqradda 4.5.3 ee GB/T27983-2011, ka dibna samee tillaabooyinka soo socda sida ku cad qodobka 4.5.3 "Kuleylka si aad u milmi ka dibna u qaboojiso" GB/T27983-2011. Samee tijaabada maran isku mar.
4. Go'aaminta wadarta guud ee macdanta birta
4.1 Mabda'a go'aaminta wuxuu la mid yahay faqradda 4.4.1 ee GB/T 21996-2008.
4.2. Reagents & Xalka
4.2.1 Aashito isku dhafan: Ku dar 150mL oo sulfuric acid ah iyo 150mL oo ah aashitada fosfooraska ilaa 700mL oo biyo ah oo si fiican isku qas.
4.2.2 Sodium diphenylamine sulfonate tusaha xalka: 5g/L, loo diyaariyey si waafaqsan GB/T603.
4.2.3 Cerium sulfate xal titration heerka: fiirsashada c [Ce (SO4) 2] = 0.1 mol/L, loo diyaariyey si waafaqsan GB/T601.
4.3 Tallaabooyinka falanqaynta
Samee laba tijaabo oo isbarbar socda. Miisaanka 0.1g oo muunad ah, oo sax ah 020001g, ku rid weel kooneedka 250ml, ku dar 10ml oo aashito ah oo isku qasan,kadib milanka,ku dar 30ml oo biyo ah iyo 4 dhibcood oo sodium dianiline sulfonate solution ah,kadib samee tillaabooyinka soo socda sida waafaqsan qodobka 4.4.2 ee GB/T209. Samee tijaabada maran isku mar.
4.4 Matalaadda natiijooyinka
Isku geynta xadiidka X1 ee isku dhafka birta amino acid marka loo eego jajabka tirada badan ee birta, qiimaha lagu muujiyey%, waxaa loo xisaabiyey hab waafaqsan qaacidada (1):
X1=(V-V0)×C×M×10-3×100
Qaabka: V - mugga cerium sulfate xalka caadiga ah ee loo isticmaalo titration ee xalka tijaabada, mL;
V0 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration of xal madhan, mL;
C - Xalka saxda ah ee cerium sulfate ee xalka caadiga ah, mol/L
5. Xisaabinta macdanta birta ee chelates
Maaddada birta ah ee X2 ee chelate marka loo eego jajabka tirada badan ee birta, qiimaha lagu sheegay%, waxaa loo xisaabiyay si waafaqsan qaacidada: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Qaabka: V1 - mugga cerium sulfate xalka caadiga ah ee loo isticmaalo titration of xalka tijaabada, mL;
V2 - xalka caadiga ah ee cerium sulfate ee loo isticmaalo titration of xal madhan, mL;
C - Xalka saxda ah ee cerium sulfate xalinta caadiga ah, mol/L;
0.05585 - cufnaanta birta birta ah ee lagu muujiyey garaam oo u dhiganta 1.00 mL ee cerium sulfate solution standard C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Cirmiga shaybaarka, g. U qaado celceliska xisaabinta natiijooyinka go'aaminta isbarbar-dhigga sida natiijooyinka go'aaminta, iyo farqiga dhammaystiran ee natiijooyinka go'aaminta barbar-dhigga ah kama badna 0.3%.
6. Xisaabinta heerka chelation
Heerka Chelation X3, qiimaha lagu muujiyey %, X3 = X2/X1 × 100
Lifaaqa C: Hababka lagu go'aamiyo heerka chelation ee Zinpro
Qaadashada heerka: Q/320205 KAVNO7-2016
1. Reagents iyo alaabta
a) Glacial acetic acid: falanqayn ahaan saafi ah; b) Perchloric acid: 0.0500mol/L; c) Tusiyaha: 0.1% tilmaame violet crystal (glacial acetic acid)
2. Go'aaminta amino acids-ka bilaashka ah
2.1 Tijaabooyinku waxa lagu qallajiyey 80°C 1 saac.
2.2 Ku rid muunada weel qalalan si aad si dabiici ah ugu qaboojiso heerkulka qolka ama aad u qaboojiso heerkul la isticmaali karo.
2.3 Miisaanka qiyaastii 0.1 g oo muunad ah (sax ah ilaa 0.001 g) oo gal 250 mL weel qallalan
2.4 Si dhakhso leh ugu gudub talaabada xigta si aad uga fogaato muunada inay nuugo qoyaanka deegaanka.
2.5 Ku dar 25mL oo ah glacial acetic acid oo si fiican isku qas wax aan ka badnayn 5min.
2.6 Ku dar 2 dhibcood oo tilmaame violet crystal ah.
2.7 Titrate leh 0.0500mol/L (± 0.001) xal titration caadiga ah ee perchloric acid ilaa uu xalku ka bedelayo guduudan ilaa cagaar 15s iyada oo aan midabka beddelin sida barta dhamaadka.
2.8 Qor mugga xalalka caadiga ah ee la isticmaalo.
2.9 Samee tijaabada maran isku mar.
3. Xisaabinta iyo natiijooyinka
Maadada amino acid-ka ee bilaashka ah ee ku jirta reagent-ka waxa loo muujiyaa sida jajab tiro badan (%), oo loo xisaabiyay hab waafaqsan qaacidada (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
Qaaciddada: C --fiirsashada xalalka perchloric acid ee caadiga ah ee moles halkii litir (mol/L)
V1 - mugga loo isticmaalo titration of shaybaarka leh xal perchloric acid caadiga ah, millilitir (ml).
Vo - mugga loo isticmaalo titration maran oo leh xalka perchloric acid caadiga ah, millilitir (mL);
M - Cufka muunada, garaam (g).
0.1445 - Celceliska tirada amino acids ee u dhiganta 1.00 mL ee xalalka perchloric acid caadiga ah [c (HClO4) = 1.000 mol / L].
4. Xisaabinta heerka chelation
Qiyaasta chelation ee muunada waxa lagu muujiyay jajab tiro badan (%), oo loo xisaabiyay si waafaqsan qaacidada (2): heerka chelation = (wadarta amino acid - ka kooban amino acid bilaash ah)/wadarta guud ee ka kooban amino acid×100%.
Waqtiga boostada: Seb-17-2025
